Crystallization and preliminary X-ray crystallographic analysis of UDP-glucuronic acid:flavonol-3-O-glucuronosyltransferase (VvGT5) from the grapevine Vitis vinifera

Grapevine (Vitis vinifera) glycosyltransferase 5 (VvGT5) is a UDP‐glucuronic acid:flavonol‐3‐O‐glucuronosyltransferase that catalyses the 3‐O‐specific glucuronosylation of flavonols using UDP‐glucuronic acid as a sugar donor to produce flavonol 3‐O‐glucosides, which are important bioactive phytochem...

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Published in:Acta crystallographica. Section F, Structural biology and crystallization communications Structural biology and crystallization communications, 2013-01, Vol.69 (1), p.65-68
Main Authors: Mizohata, Eiichi, Okuda, Takuma, Hatanaka, Seika, Nakayama, Taisuke, Horikawa, Manabu, Nakayama, Toru, Ono, Eiichiro, Inoue, Tsuyoshi
Format: Article
Language:English
Subjects:
Crystallization
Crystallization - methods
Crystallization Communications
Crystallography
Crystallography, X-Ray
Diffraction
E coli
Escherichia coli
Escherichia coli - genetics
flavonoid
flavonol
Flavonols
Glucosyltransferases - chemistry
Glucosyltransferases - genetics
Glucosyltransferases - isolation & purification
glycosyltransferase
Grapevines
Minerals
Phases
Plant Proteins - chemistry
Plant Proteins - genetics
Plant Proteins - isolation & purification
Protein Conformation
Recombinant
Recombinant Proteins - chemistry
Recombinant Proteins - genetics
Recombinant Proteins - isolation & purification
UGT
Vitis - enzymology
VvGT5
X-ray diffraction
X-rays
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Summary:Grapevine (Vitis vinifera) glycosyltransferase 5 (VvGT5) is a UDP‐glucuronic acid:flavonol‐3‐O‐glucuronosyltransferase that catalyses the 3‐O‐specific glucuronosylation of flavonols using UDP‐glucuronic acid as a sugar donor to produce flavonol 3‐O‐glucosides, which are important bioactive phytochemicals. Recombinant VvGT5 expressed in Escherichia coli cells was purified and crystallized by the sitting‐drop vapour‐diffusion method. A full set of X‐ray diffraction data was collected to 2.2 Å Bragg spacing from a single crystal using a synchrotron‐radiation source. The crystal was hexagonal, belonging to space group P6122, with unit‐cell parameters a = b = 102.70, c = 535.92 Å. The initial phases were determined by the molecular‐replacement method.
ISSN:1744-3091
1744-3091
2053-230X
DOI:10.1107/S1744309112045095