Decreased afferent excitability contributes to synaptic depression during high-frequency stimulation in hippocampal area CA1

• Published in: Journal of neurophysiology 2012-10, Vol.108 (7), p.1965-1976
• Main Authors: Kim, Eunyoung, Owen, Benjamin, Holmes, William R, Grover, Lawrence M
• Format: Article
• Language: English
• Subjects: Action Potentials; Afferent Pathways - physiology; Animals; CA1 Region, Hippocampal - physiology; CA3 Region, Hippocampal - physiology; Calcium - metabolism; Electric Stimulation; Excitatory Postsynaptic Potentials; Long-Term Synaptic Depression - physiology; Male; Pyramidal Cells - physiology; Rats; Rats, Sprague-Dawley; Synaptic Vesicles - metabolism
• ISSN: 0022-3077; 1522-1598
• DOI: 10.1152/jn.00276.2011

Long-term potentiation (LTP) is often induced experimentally by continuous high-frequency afferent stimulation (HFS), typically at 100 Hz for 1 s. Induction of LTP requires postsynaptic depolarization and voltage-dependent calcium influx. Induction is more effective if the same number of stimuli are given as a series of short bursts rather than as continuous HFS, in part because excitatory postsynaptic potentials (EPSPs) become strongly depressed during HFS, reducing postsynaptic depolarization. In this study, we examined mechanisms of EPSP depression during HFS in area CA1 of rat hippocampal brain slices. We tested for presynaptic terminal vesicle depletion by examining minimal stimulation-evoked excitatory postsynaptic currents (EPSCs) during 100-Hz HFS. While transmission failures increased, consistent with vesicle depletion, EPSC latencies also increased during HFS, suggesting a decrease in afferent excitability. Extracellular recordings of Schaffer collateral fiber volleys confirmed a decrease in afferent excitability, with decreased fiber volley amplitudes and increased latencies during HFS. To determine the mechanism responsible for fiber volley changes, we recorded antidromic action potentials in single CA3 pyramidal neurons evoked by stimulating Schaffer collateral axons. During HFS, individual action potentials decreased in amplitude and increased in latency, and these changes were accompanied by a large increase in the probability of action potential failure. Time derivative and phase-plane analyses indicated decreases in both axon initial segment and somato-dendritic components of CA3 neuron action potentials. Our results indicate that decreased presynaptic axon excitability contributes to depression of excitatory synaptic transmission during HFS at synapses between Schaffer collaterals and CA1 pyramidal neurons.