Multinozzle Emitter Array Chips for Small-Volume Proteomics

High-throughput multiplexed proteomics of small-volume biospecimens will generate new opportunities in theranostics. Achieving parallel top-down and bottom-up mass spectrometry analyses of target proteins using a unified apparatus will improve proteome characterization. We have developed a novel sil...

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Bibliographic Details
Published in:Analytical chemistry (Washington) 2013-01, Vol.85 (2), p.816-819
Main Authors: Mao, Pan, Gomez-Sjoberg, Rafael, Wang, Daojing
Format: Article
Language:English
Subjects:
Analytical chemistry
Arrays
Chromatography
Chromatography, Liquid
detection limit
erythrocytes
hemoglobin
Hemoglobins - analysis
Humans
liquid chromatography
Mass spectrometry
Microfluidic Analytical Techniques - instrumentation
Proteins
proteome
Proteomics
Silicon - chemistry
Spectrometry, Mass, Electrospray Ionization
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Summary:High-throughput multiplexed proteomics of small-volume biospecimens will generate new opportunities in theranostics. Achieving parallel top-down and bottom-up mass spectrometry analyses of target proteins using a unified apparatus will improve proteome characterization. We have developed a novel silicon-based microfluidic device, multinozzle emitter array chip (MEA chip), as a new platform for small-volume proteomics using liquid chromatography-nanoelectrospray ionization mass spectrometry (LC-nanoESI-MS). We demonstrate parallel, on-chip, and online LC-MS analysis of hemoglobin and its tryptic digests directly from microliters of blood, achieving a detection limit of less than 5 red blood cells. Our MEA chip will enable clinical proteomics of small-volume samples.
ISSN:0003-2700
1520-6882
1520-6882
DOI:10.1021/ac3032965