Multinozzle Emitter Array Chips for Small-Volume Proteomics

High-throughput multiplexed proteomics of small-volume biospecimens will generate new opportunities in theranostics. Achieving parallel top-down and bottom-up mass spectrometry analyses of target proteins using a unified apparatus will improve proteome characterization. We have developed a novel sil...

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Published in:Analytical chemistry (Washington) 2013-01, Vol.85 (2), p.816-819
Main Authors: Mao, Pan, Gomez-Sjoberg, Rafael, Wang, Daojing
Format: Article
Language:English
Subjects:
Analytical chemistry
Arrays
Chromatography
Chromatography, Liquid
detection limit
erythrocytes
hemoglobin
Hemoglobins - analysis
Humans
liquid chromatography
Mass spectrometry
Microfluidic Analytical Techniques - instrumentation
Proteins
proteome
Proteomics
Silicon - chemistry
Spectrometry, Mass, Electrospray Ionization
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cited_by cdi_FETCH-LOGICAL-a466t-4c13e65a5125d8f0d80527bad8ad02557adba7146b2642b723b150a5f758d3223
cites cdi_FETCH-LOGICAL-a466t-4c13e65a5125d8f0d80527bad8ad02557adba7146b2642b723b150a5f758d3223
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container_title Analytical chemistry (Washington)
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creator Mao, Pan
Gomez-Sjoberg, Rafael
Wang, Daojing
description High-throughput multiplexed proteomics of small-volume biospecimens will generate new opportunities in theranostics. Achieving parallel top-down and bottom-up mass spectrometry analyses of target proteins using a unified apparatus will improve proteome characterization. We have developed a novel silicon-based microfluidic device, multinozzle emitter array chip (MEA chip), as a new platform for small-volume proteomics using liquid chromatography-nanoelectrospray ionization mass spectrometry (LC-nanoESI-MS). We demonstrate parallel, on-chip, and online LC-MS analysis of hemoglobin and its tryptic digests directly from microliters of blood, achieving a detection limit of less than 5 red blood cells. Our MEA chip will enable clinical proteomics of small-volume samples.
doi_str_mv 10.1021/ac3032965
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source American Chemical Society:Jisc Collections:American Chemical Society Read & Publish Agreement 2022-2024 (Reading list)
subjects Analytical chemistry
Arrays
Chromatography
Chromatography, Liquid
detection limit
erythrocytes
hemoglobin
Hemoglobins - analysis
Humans
liquid chromatography
Mass spectrometry
Microfluidic Analytical Techniques - instrumentation
Proteins
proteome
Proteomics
Silicon - chemistry
Spectrometry, Mass, Electrospray Ionization
title Multinozzle Emitter Array Chips for Small-Volume Proteomics
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