TRPV1: Contribution to Retinal Ganglion Cell Apoptosis and Increased Intracellular Ca2+ with Exposure to Hydrostatic Pressure

Elevated hydrostatic pressure induces retinal ganglion cell (RGC) apoptosis in culture. The authors investigated whether the transient receptor potential vanilloid 1 (TRPV1) channel, which contributes to pressure sensing and Ca(2+)-dependent cell death in other systems, also contributes to pressure-...

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Bibliographic Details
Published in:Investigative ophthalmology & visual science 2009-02, Vol.50 (2), p.717-728
Main Authors: Sappington, Rebecca M, Sidorova, Tatiana, Long, Daniel J, Calkins, David J
Format: Article
Language:English
Subjects:
Animals
Apoptosis
Axons - metabolism
Biological and medical sciences
Blotting, Western
Calcium - metabolism
Diterpenes - pharmacology
Eye and associated structures. Visual pathways and centers. Vision
Fundamental and applied biological sciences. Psychology
Hydrostatic Pressure
In Situ Hybridization
Intraocular Pressure
Medical sciences
Mice
Mice, Inbred C57BL
Mice, Inbred DBA
Microscopy, Fluorescence
Ophthalmology
Rats
Rats, Sprague-Dawley
Retinal Ganglion Cells - metabolism
Retinal Ganglion Cells - pathology
Reverse Transcriptase Polymerase Chain Reaction
RNA, Messenger - metabolism
TRPV Cation Channels - antagonists & inhibitors
TRPV Cation Channels - physiology
Vertebrates: nervous system and sense organs
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Summary:Elevated hydrostatic pressure induces retinal ganglion cell (RGC) apoptosis in culture. The authors investigated whether the transient receptor potential vanilloid 1 (TRPV1) channel, which contributes to pressure sensing and Ca(2+)-dependent cell death in other systems, also contributes to pressure-induced RGC death and whether this contribution involves Ca(2+). trpv1 mRNA expression in RGCs was probed with the use of PCR and TRPV1 protein localization through immunocytochemistry. Subunit-specific antagonism (iodo-resiniferatoxin) and agonism (capsaicin) were used to probe how TRPV1 activation affects the survival of isolated RGCs at ambient and elevated hydrostatic pressure (+70 mm Hg). Finally, for RGCs under pressure, the authors tested whether EGTA chelation of Ca(2+) improves survival and whether, with the Ca(2+) dye Fluo-4 AM, TRPV1 contributes to increased intracellular Ca(2+). RGCs express trpv1 mRNA, with robust TRPV1 protein localization to the cell body and axon. For isolated RGCs under pressure, TRPV1 antagonism increased cell density and reduced apoptosis to ambient levels (P
ISSN:0146-0404
1552-5783
DOI:10.1167/iovs.08-2321