Comparative Analysis of Routine Laboratory Diagnostic Tests for Rabies

Present study was undertaken to compare various routine laboratory diagnostic tests for rabies detection. Seller’s staining, mouse inoculation test (MIT), Dot-ELISA, Agar gel precipitation test (AGPT) and counter immunoelectrophoresis test (CIET) were the main basic tests performed in the laboratory...

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Bibliographic Details
Published in:Indian Journal of Virology 2011-12, Vol.22 (2), p.142-145
Main Authors: Kadam, S. S., Sherikar, A. A., Pingale, V. S.
Format: Article
Language:English
Subjects:
Animals
Antibodies
Antigens
Bats
Biochemistry
Biomedical and Life Sciences
Brain
Cell Biology
Comparative analysis
Counter immunoelectrophoresis
Diagnostic tests
Enzyme-linked immunosorbent assay
Enzymes
Gel precipitation
Inoculation
Laboratories
Life Sciences
Low income groups
Lyssavirus
Medical research
Microbiology
Microscopy
Negri bodies
Protein Structure
Rabies
Sensitivity analysis
Short Communication
Stains & staining
Viral infections
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Summary:Present study was undertaken to compare various routine laboratory diagnostic tests for rabies detection. Seller’s staining, mouse inoculation test (MIT), Dot-ELISA, Agar gel precipitation test (AGPT) and counter immunoelectrophoresis test (CIET) were the main basic tests performed in the laboratory for the rabies diagnosis. Out of 200 brain specimens, Negri bodies were observed in 52 brain samples by Seller’s staining. Rabies virus was isolated in 56 samples by intra-cerebral inoculation in newborn Swiss-albino mice. Dot-ELISA and AGPT could detect rabies antigen in 55 and 57 samples respectively. Comparative analysis revealed that the CIET is the most sensitive and rapid test among performed diagnostic tests.
ISSN:0970-2822
2347-3584
0974-0120
2347-3517
DOI:10.1007/s13337-011-0052-1