Detection of Corchorusgolden mosaic virus Associated with Yellow Mosaic Disease of Jute (Corchorus capsularis)

Yellow mosaic disease, caused by a whitefly transmitted New World Begomovirus , named Corchorus golden mosaic virus (CoGMV), is emerging as a serious biotic constraint for jute fibre production in Asia. For rapid and sensitive diagnosis of the Begomovirus associated with this disease, a non-radiolab...

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Bibliographic Details
Published in:Indian Journal of Virology 2012-06, Vol.23 (1), p.70-74
Main Authors: Ghosh, Raju, Palit, Paramita, Paul, Sujay, Ghosh, Subrata Kumar, Roy, Anirban
Format: Article
Language:English
Subjects:
Begomovirus
Biochemistry
Biomedical and Life Sciences
Cell Biology
Cloning
Disease
DNA viruses
Flowers & plants
Genomes
Hybridization
Life Sciences
Microbiology
Mosaic disease
Plant viruses
Polymerase chain reaction
Protein Structure
Proteins
Short Communication
Viruses
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Summary:Yellow mosaic disease, caused by a whitefly transmitted New World Begomovirus , named Corchorus golden mosaic virus (CoGMV), is emerging as a serious biotic constraint for jute fibre production in Asia. For rapid and sensitive diagnosis of the Begomovirus associated with this disease, a non-radiolabelled diagnostic probe, developed against the DNA A component of the east Indian isolate of CoGMV, detected the presence of the virus in infected plants and viruliferous whiteflies following Southern hybridization and nucleic acid spot hybridization tests. Presence of the virus was also confirmed when polymerase chain reaction amplification was performed using virus-specific primers on DNA templates isolated from infected plants and viruliferous whiteflies.
ISSN:0970-2822
2347-3584
0974-0120
2347-3517
DOI:10.1007/s13337-012-0062-7