Radiation induced COX-2 expression and mutagenesis at non-targeted lung tissues of gpt delta transgenic mice

Background: Although radiation-induced bystander effects have been confirmed using a variety of endpoints, the mechanism(s) underlying these effects are not well understood, especially for in vivo study. Methods: A 1-cm 2 area (1 cm × 1 cm) in the lower abdominal region of gpt delta transgenic mice...

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Bibliographic Details
Published in:British journal of cancer 2013-01, Vol.108 (1), p.91-98
Main Authors: Chai, Y, Calaf, G M, Zhou, H, Ghandhi, S A, Elliston, C D, Wen, G, Nohmi, T, Amundson, S A, Hei, T K
Format: Article
Language:English
Subjects:
631/250/127/1219
631/61/191/1908
631/67/1059/485
Abdomen
Abdomen - radiation effects
Animals
Apoptosis
Apoptosis - radiation effects
Biological and medical sciences
Biomedical and Life Sciences
Biomedicine
Bystander Effect
Cancer
Cancer Research
Cyclooxygenase 2 - metabolism
Cyclooxygenase 2 - radiation effects
Cytokines
Dinoprostone - metabolism
DNA Damage
Drug Resistance
Epidemiology
Escherichia coli Proteins - genetics
Female
Growth factors
Health sciences
Liver - radiation effects
Lung - radiation effects
Lungs
Male
Medical research
Medical sciences
Mice
Mice, Transgenic
Molecular Diagnostics
Molecular Medicine
Mutagenesis
Mutation
Oncology
Pentosyltransferases - genetics
Radiation
Signal transduction
Transgenic animals
Tumors
X-Rays
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Summary:Background: Although radiation-induced bystander effects have been confirmed using a variety of endpoints, the mechanism(s) underlying these effects are not well understood, especially for in vivo study. Methods: A 1-cm 2 area (1 cm × 1 cm) in the lower abdominal region of gpt delta transgenic mice was irradiated with 5 Gy of 300 keV X-rays, and changes in out-of-field lung and liver were observed. Results: Compared with sham-treated controls, the Spi − mutation frequency increased 2.4-fold in non-targeted lung tissues at 24 h after partial body irradiation (PBIR). Consistent with dramatic Cyclooxygenase 2 (COX-2) induction in the non-targeted bronchial epithelial cells, increasing levels of prostaglandin, together with 8-hydroxydeoxyguanosine, in the out-of-field lung tissues were observed after PBIR. In addition, DNA double-strand breaks and apoptosis were induced in bystander lung tissues after PBIR. Conclusion: The PBIR induces DNA damage and mutagenesis in non-targeted lung tissues, especially in bronchial epithelial cells, and COX-2 has an essential role in bystander mutagenesis.
ISSN:0007-0920
1532-1827
DOI:10.1038/bjc.2012.498