role for the essential YycG sensor histidine kinase in sensing cell division

The YycG sensor histidine kinase co-ordinates cell wall remodelling with cell division in Gram-positive bacteria by controlling the transcription of genes for autolysins and their inhibitors. Bacillus subtilis YycG senses cell division and is enzymatically activated by associating with the divisome...

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Bibliographic Details
Published in:Molecular microbiology 2011-01, Vol.79 (2), p.503-522
Main Authors: Fukushima, Tatsuya, Furihata, Isako, Emmins, Robyn, Daniel, Richard A, Hoch, James A, Szurmant, Hendrik
Format: Article
Language:English
Subjects:
Amino acids
Bacillus subtilis - physiology
Biological and medical sciences
Cell Division
Enzyme kinetics
Fundamental and applied biological sciences. Psychology
Genes
Histidine Kinase
Kinases
Microbiology
Molecules
Protein Interaction Domains and Motifs
Protein Interaction Mapping
Protein Kinases - genetics
Protein Kinases - metabolism
Sequence Deletion
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Summary:The YycG sensor histidine kinase co-ordinates cell wall remodelling with cell division in Gram-positive bacteria by controlling the transcription of genes for autolysins and their inhibitors. Bacillus subtilis YycG senses cell division and is enzymatically activated by associating with the divisome at the division septum. Here it is shown that the cytoplasmic PAS domain of this multi-domain transmembrane kinase is a determining factor translocating the kinase to the division septum. Furthermore, translocation to the division septum, per se, is insufficient to activate YycG, indicating that specific interactions and/or ligands produced there are required to stimulate kinase activity. N-terminal truncations of YycG lose negative regulation of their activity inferring that this regulation is accomplished through its transmembrane and extramembrane domains interacting with the membrane associated YycH and YycI proteins that do not localize to the divisome. The data indicate that YycG activity in non-dividing cells is suppressed by its interaction with YycH and YycI and its activation is co-ordinated to cell division in dividing cells by specific interactions that occur within the divisome.
ISSN:0950-382X
1365-2958
DOI:10.1111/j.1365-2958.2010.07464.x