Increased neuronal activity fragments the Golgi complex

The Golgi complex is essential for many aspects of cellular function, including trafficking and sorting of membrane and secretory proteins and posttranslational modification by glycosylation. We observed reversible fragmentation of the Golgi complex in cultured hippocampal neurons cultured in hypere...

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Bibliographic Details
Published in:Proceedings of the National Academy of Sciences - PNAS 2013-01, Vol.110 (4), p.1482-1487
Main Authors: Thayer, Desiree A., Jan, Yuh Nung, Jan, Lily Yeh
Format: Article
Language:English
Subjects:
2-Amino-5-phosphonovalerate - pharmacology
Animals
Apoptosis
Benzylamines - pharmacology
Bicuculline - pharmacology
Biological and medical sciences
Biological Sciences
Calcium
Calcium Signaling
Calcium-Calmodulin-Dependent Protein Kinases - antagonists & inhibitors
Calcium-Calmodulin-Dependent Protein Kinases - metabolism
Cells
Cells, Cultured
Disease models
Electrophysiological Phenomena
Enzyme Activation
Fundamental and applied biological sciences. Psychology
GABA-A Receptor Antagonists - pharmacology
Glycosylation
Golgi apparatus
Golgi Apparatus - drug effects
Golgi Apparatus - physiology
Golgi Apparatus - ultrastructure
Hyperactivity
Nerve Tissue Proteins - metabolism
Neurons
Neurons - drug effects
Neurons - physiology
Neuropsychology
Potassium
Potassium - pharmacology
Proteins
Rats
Receptors, N-Methyl-D-Aspartate - antagonists & inhibitors
Signal transduction
Statistical median
Sulfonamides - pharmacology
Surface areas
Vertebrates: nervous system and sense organs
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Summary:The Golgi complex is essential for many aspects of cellular function, including trafficking and sorting of membrane and secretory proteins and posttranslational modification by glycosylation. We observed reversible fragmentation of the Golgi complex in cultured hippocampal neurons cultured in hyperexcitable conditions. In addition, Golgi fragmentation was found in cultured neurons with hyperactivity due to prolonged blockade of GABA A-mediated inhibition or withdrawal of NMDA receptor antagonism. The interplay between neuronal hyperactivity and Golgi structure established in this study thus reveals a previously uncharacterized impact of neuronal activity on organelle structure. This finding may have important roles in protein processing and trafficking in the Golgi as well as effects on neuronal signaling.
ISSN:0027-8424
1091-6490
DOI:10.1073/pnas.1220978110