Covalently Linked Tandem Lesions in DNA

Reactive oxygen species (ROS) generate a type of DNA damage called tandem lesions, two adjacent nucleotides both modified. A subcategory of tandem lesions consists of adjacent nucleotides linked by a covalent bond. Covalently linked tandem lesions generate highly characteristic liquid chromotography...

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Bibliographic Details
Published in:Radiation research 2012-12, Vol.178 (6), p.538-542
Main Authors: Patrzyc, Helen B., Dawidzik, Jean B., Budzinski, Edwin E., Freund, Harold G., Wilton, John H., Box, Harold C.
Format: Article
Language:English
Subjects:
Animals
Cattle
Deoxyribonucleic acid
DNA
DNA - chemistry
DNA - genetics
DNA - metabolism
DNA Damage
Elution
Ions
Lesions
Mass spectrometry
Mass spectroscopy
Molecular weight
Nucleotides
Oligomers
Oxygen
Oxygen - metabolism
REGULAR ARTICLES
Space life sciences
X-Rays - adverse effects
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Summary:Reactive oxygen species (ROS) generate a type of DNA damage called tandem lesions, two adjacent nucleotides both modified. A subcategory of tandem lesions consists of adjacent nucleotides linked by a covalent bond. Covalently linked tandem lesions generate highly characteristic liquid chromotography-tandem mass spectrometry (LC-MS/MS) elution profiles. We have used this property to comprehensively survey X-irradiated DNA for covalently linked tandem lesions. A total of 15 tandem lesions were detected in DNA irradiated in deoxygenated aqueous solution, five tandem lesions were detected in DNA that was irradiated in oxygenated solution.
ISSN:0033-7587
1938-5404
DOI:10.1667/RR2915.1