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Comparison of the myocardial clearance of endothelial progenitor cells injected early versus late into reperfused or sustained occlusion myocardial infarction
Stem cell transplantation following AMI has shown promise for the repair or reduction of the amount of myocardial injury. There is some evidence that these treatment effects appear to be directly correlated to cell residence time. This study aims to assess the effects of (a) the timing of stem cell...
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Published in: | The International Journal of Cardiovascular Imaging 2013-02, Vol.29 (2), p.497-504 |
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description | Stem cell transplantation following AMI has shown promise for the repair or reduction of the amount of myocardial injury. There is some evidence that these treatment effects appear to be directly correlated to cell residence time. This study aims to assess the effects of (a) the timing of stem cell injection following myocardial infarction, and (b) flow milieu, on cell residence times at the site of transplantation by comparing three time points (day of infarction, week 1 and week 4–5), and two models of acute myocardial infarction (sustained occlusion or reperfusion). Twenty-one dogs received 2 injections of 30 million endothelial progenitor cells. The first injections were administered by epicardial (n = 8) or endocardial injection (n = 13) either on the day of infarction (n = 15) or at 1 week (n = 6). The second injections were administered by only endocardial injection (n = 18) 4 weeks following the first injection. Cell clearance half-lives were comparable between early and late injections. However, transplants into sustained occlusion infarcts resulted in slower cell clearance 77.1 ± 6.1 (n = 18) versus reperfused 59.4 ± 2.9 h (n = 21)
p
= 0.009. Sustained occlusion infarcts had longer cell retention in comparison to reperfusion whereas the timing of injection did not affect clearance rates. If the potential for myocardial regeneration associated with cell transplantation is, at least in part, linked to cell residence times, then greater benefit may be observed with transplants into infarcts associated with persistent coronary artery occlusion. |
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p
= 0.009. Sustained occlusion infarcts had longer cell retention in comparison to reperfusion whereas the timing of injection did not affect clearance rates. If the potential for myocardial regeneration associated with cell transplantation is, at least in part, linked to cell residence times, then greater benefit may be observed with transplants into infarcts associated with persistent coronary artery occlusion.</description><identifier>ISSN: 1569-5794</identifier><identifier>EISSN: 1573-0743</identifier><identifier>EISSN: 1875-8312</identifier><identifier>DOI: 10.1007/s10554-012-0086-5</identifier><identifier>PMID: 22736429</identifier><identifier>CODEN: IJCIBI</identifier><language>eng</language><publisher>Dordrecht: Springer Netherlands</publisher><subject>Animals ; Cardiac Imaging ; Cardiology ; Cell Survival ; Cell Tracking ; Disease Models, Animal ; Dogs ; Endothelial Cells - pathology ; Endothelial Cells - transplantation ; Female ; Imaging ; Medicine ; Medicine & Public Health ; Multimodal Imaging ; Myocardial Infarction - diagnostic imaging ; Myocardial Infarction - pathology ; Myocardial Infarction - surgery ; Myocardial Reperfusion Injury - diagnostic imaging ; Myocardial Reperfusion Injury - pathology ; Myocardial Reperfusion Injury - surgery ; Myocardium - pathology ; Original Paper ; Positron-Emission Tomography ; Radiology ; Stem Cell Transplantation ; Time Factors ; Tomography, X-Ray Computed</subject><ispartof>The International Journal of Cardiovascular Imaging, 2013-02, Vol.29 (2), p.497-504</ispartof><rights>The Author(s) 2012</rights><rights>Springer Science+Business Media Dordrecht 2013</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c470t-b021fbb4e4a799cfb1fa8848e7fa0cd3e33c9866e0b4bc5a05c357ffc82550b23</citedby><cites>FETCH-LOGICAL-c470t-b021fbb4e4a799cfb1fa8848e7fa0cd3e33c9866e0b4bc5a05c357ffc82550b23</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>230,314,780,784,885,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/22736429$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Mitchell, Andrea J.</creatorcontrib><creatorcontrib>Sabondjian, Eric</creatorcontrib><creatorcontrib>Blackwood, Kimberley J.</creatorcontrib><creatorcontrib>Sykes, Jane</creatorcontrib><creatorcontrib>Deans, Lela</creatorcontrib><creatorcontrib>Feng, Qingping</creatorcontrib><creatorcontrib>Stodilka, Robert Z.</creatorcontrib><creatorcontrib>Prato, Frank S.</creatorcontrib><creatorcontrib>Wisenberg, Gerald</creatorcontrib><title>Comparison of the myocardial clearance of endothelial progenitor cells injected early versus late into reperfused or sustained occlusion myocardial infarction</title><title>The International Journal of Cardiovascular Imaging</title><addtitle>Int J Cardiovasc Imaging</addtitle><addtitle>Int J Cardiovasc Imaging</addtitle><description>Stem cell transplantation following AMI has shown promise for the repair or reduction of the amount of myocardial injury. There is some evidence that these treatment effects appear to be directly correlated to cell residence time. This study aims to assess the effects of (a) the timing of stem cell injection following myocardial infarction, and (b) flow milieu, on cell residence times at the site of transplantation by comparing three time points (day of infarction, week 1 and week 4–5), and two models of acute myocardial infarction (sustained occlusion or reperfusion). Twenty-one dogs received 2 injections of 30 million endothelial progenitor cells. The first injections were administered by epicardial (n = 8) or endocardial injection (n = 13) either on the day of infarction (n = 15) or at 1 week (n = 6). The second injections were administered by only endocardial injection (n = 18) 4 weeks following the first injection. Cell clearance half-lives were comparable between early and late injections. However, transplants into sustained occlusion infarcts resulted in slower cell clearance 77.1 ± 6.1 (n = 18) versus reperfused 59.4 ± 2.9 h (n = 21)
p
= 0.009. Sustained occlusion infarcts had longer cell retention in comparison to reperfusion whereas the timing of injection did not affect clearance rates. If the potential for myocardial regeneration associated with cell transplantation is, at least in part, linked to cell residence times, then greater benefit may be observed with transplants into infarcts associated with persistent coronary artery occlusion.</description><subject>Animals</subject><subject>Cardiac Imaging</subject><subject>Cardiology</subject><subject>Cell Survival</subject><subject>Cell Tracking</subject><subject>Disease Models, Animal</subject><subject>Dogs</subject><subject>Endothelial Cells - pathology</subject><subject>Endothelial Cells - transplantation</subject><subject>Female</subject><subject>Imaging</subject><subject>Medicine</subject><subject>Medicine & Public Health</subject><subject>Multimodal Imaging</subject><subject>Myocardial Infarction - diagnostic imaging</subject><subject>Myocardial Infarction - pathology</subject><subject>Myocardial Infarction - surgery</subject><subject>Myocardial Reperfusion Injury - diagnostic imaging</subject><subject>Myocardial Reperfusion Injury - pathology</subject><subject>Myocardial Reperfusion Injury - surgery</subject><subject>Myocardium - pathology</subject><subject>Original Paper</subject><subject>Positron-Emission Tomography</subject><subject>Radiology</subject><subject>Stem Cell Transplantation</subject><subject>Time Factors</subject><subject>Tomography, X-Ray Computed</subject><issn>1569-5794</issn><issn>1573-0743</issn><issn>1875-8312</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2013</creationdate><recordtype>article</recordtype><recordid>eNp1Uc1u1DAQjhCIlsIDcEGRuHAJjO04Ti5IaMWfVIkLnC3HGW-9cuxgO5X2ZfqsOGypFiROtuf7mRl_VfWSwFsCIN4lApy3DRDaAPRdwx9Vl4QL1oBo2ePt3g0NF0N7UT1L6QAAFCh7Wl1QKljX0uGyutuFeVHRpuDrYOp8g_V8DFrFySpXa4cqKq9xw9BPoeBuA5YY9uhtDrHW6FyqrT-gzjjVReCO9S3GtKbaqYwFyqGOuGA0ayqMoilYVtZvD63dmmzpftbWeqOizqX6vHpilEv44v68qn58-vh996W5_vb56-7DdaNbAbkZgRIzji22SgyDNiMxqu_bHoVRoCeGjOmh7zqEsR01V8A148IY3VPOYaTsqnp_8l3WccZJo89ROblEO6t4lEFZ-Tfi7Y3ch1vJeAcD74rBm3uDGH6umLKcbdq-RnkMa5KE9kzQnjBWqK__oR7CGn1Z7zeLsIH120TkxNIxpBTRPAxDQG7py1P6sqQvt_QlL5pX51s8KP7EXQj0REgF8nuMZ63_6_oLi43AXQ</recordid><startdate>20130201</startdate><enddate>20130201</enddate><creator>Mitchell, Andrea J.</creator><creator>Sabondjian, Eric</creator><creator>Blackwood, Kimberley J.</creator><creator>Sykes, Jane</creator><creator>Deans, Lela</creator><creator>Feng, Qingping</creator><creator>Stodilka, Robert Z.</creator><creator>Prato, Frank S.</creator><creator>Wisenberg, Gerald</creator><general>Springer Netherlands</general><general>Springer Nature B.V</general><scope>C6C</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8FD</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>BENPR</scope><scope>CCPQU</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>K9.</scope><scope>M0S</scope><scope>M1P</scope><scope>M7Z</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20130201</creationdate><title>Comparison of the myocardial clearance of endothelial progenitor cells injected early versus late into reperfused or sustained occlusion myocardial infarction</title><author>Mitchell, Andrea J. ; Sabondjian, Eric ; Blackwood, Kimberley J. ; Sykes, Jane ; Deans, Lela ; Feng, Qingping ; Stodilka, Robert Z. ; Prato, Frank S. ; Wisenberg, Gerald</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c470t-b021fbb4e4a799cfb1fa8848e7fa0cd3e33c9866e0b4bc5a05c357ffc82550b23</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2013</creationdate><topic>Animals</topic><topic>Cardiac Imaging</topic><topic>Cardiology</topic><topic>Cell Survival</topic><topic>Cell Tracking</topic><topic>Disease Models, Animal</topic><topic>Dogs</topic><topic>Endothelial Cells - pathology</topic><topic>Endothelial Cells - transplantation</topic><topic>Female</topic><topic>Imaging</topic><topic>Medicine</topic><topic>Medicine & Public Health</topic><topic>Multimodal Imaging</topic><topic>Myocardial Infarction - diagnostic imaging</topic><topic>Myocardial Infarction - pathology</topic><topic>Myocardial Infarction - surgery</topic><topic>Myocardial Reperfusion Injury - diagnostic imaging</topic><topic>Myocardial Reperfusion Injury - pathology</topic><topic>Myocardial Reperfusion Injury - surgery</topic><topic>Myocardium - pathology</topic><topic>Original Paper</topic><topic>Positron-Emission Tomography</topic><topic>Radiology</topic><topic>Stem Cell Transplantation</topic><topic>Time Factors</topic><topic>Tomography, X-Ray Computed</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Mitchell, Andrea J.</creatorcontrib><creatorcontrib>Sabondjian, Eric</creatorcontrib><creatorcontrib>Blackwood, Kimberley J.</creatorcontrib><creatorcontrib>Sykes, Jane</creatorcontrib><creatorcontrib>Deans, Lela</creatorcontrib><creatorcontrib>Feng, Qingping</creatorcontrib><creatorcontrib>Stodilka, Robert Z.</creatorcontrib><creatorcontrib>Prato, Frank S.</creatorcontrib><creatorcontrib>Wisenberg, Gerald</creatorcontrib><collection>SpringerOpen</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Health & Medical Collection (Proquest)</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Technology Research Database</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni)</collection><collection>ProQuest Central</collection><collection>ProQuest Central</collection><collection>ProQuest One Community College</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>PML(ProQuest Medical Library)</collection><collection>Biochemistry Abstracts 1</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>The International Journal of Cardiovascular Imaging</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Mitchell, Andrea J.</au><au>Sabondjian, Eric</au><au>Blackwood, Kimberley J.</au><au>Sykes, Jane</au><au>Deans, Lela</au><au>Feng, Qingping</au><au>Stodilka, Robert Z.</au><au>Prato, Frank S.</au><au>Wisenberg, Gerald</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Comparison of the myocardial clearance of endothelial progenitor cells injected early versus late into reperfused or sustained occlusion myocardial infarction</atitle><jtitle>The International Journal of Cardiovascular Imaging</jtitle><stitle>Int J Cardiovasc Imaging</stitle><addtitle>Int J Cardiovasc Imaging</addtitle><date>2013-02-01</date><risdate>2013</risdate><volume>29</volume><issue>2</issue><spage>497</spage><epage>504</epage><pages>497-504</pages><issn>1569-5794</issn><eissn>1573-0743</eissn><eissn>1875-8312</eissn><coden>IJCIBI</coden><abstract>Stem cell transplantation following AMI has shown promise for the repair or reduction of the amount of myocardial injury. There is some evidence that these treatment effects appear to be directly correlated to cell residence time. This study aims to assess the effects of (a) the timing of stem cell injection following myocardial infarction, and (b) flow milieu, on cell residence times at the site of transplantation by comparing three time points (day of infarction, week 1 and week 4–5), and two models of acute myocardial infarction (sustained occlusion or reperfusion). Twenty-one dogs received 2 injections of 30 million endothelial progenitor cells. The first injections were administered by epicardial (n = 8) or endocardial injection (n = 13) either on the day of infarction (n = 15) or at 1 week (n = 6). The second injections were administered by only endocardial injection (n = 18) 4 weeks following the first injection. Cell clearance half-lives were comparable between early and late injections. However, transplants into sustained occlusion infarcts resulted in slower cell clearance 77.1 ± 6.1 (n = 18) versus reperfused 59.4 ± 2.9 h (n = 21)
p
= 0.009. Sustained occlusion infarcts had longer cell retention in comparison to reperfusion whereas the timing of injection did not affect clearance rates. If the potential for myocardial regeneration associated with cell transplantation is, at least in part, linked to cell residence times, then greater benefit may be observed with transplants into infarcts associated with persistent coronary artery occlusion.</abstract><cop>Dordrecht</cop><pub>Springer Netherlands</pub><pmid>22736429</pmid><doi>10.1007/s10554-012-0086-5</doi><tpages>8</tpages><oa>free_for_read</oa></addata></record> |
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subjects | Animals Cardiac Imaging Cardiology Cell Survival Cell Tracking Disease Models, Animal Dogs Endothelial Cells - pathology Endothelial Cells - transplantation Female Imaging Medicine Medicine & Public Health Multimodal Imaging Myocardial Infarction - diagnostic imaging Myocardial Infarction - pathology Myocardial Infarction - surgery Myocardial Reperfusion Injury - diagnostic imaging Myocardial Reperfusion Injury - pathology Myocardial Reperfusion Injury - surgery Myocardium - pathology Original Paper Positron-Emission Tomography Radiology Stem Cell Transplantation Time Factors Tomography, X-Ray Computed |
title | Comparison of the myocardial clearance of endothelial progenitor cells injected early versus late into reperfused or sustained occlusion myocardial infarction |
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