AT-rich sequence elements promote nascent transcript cleavage leading to RNA polymerase II termination

RNA Polymerase II (Pol II) termination is dependent on RNA processing signals as well as specific terminator elements located downstream of the poly(A) site. One of the two major terminator classes described so far is the Co-Transcriptional Cleavage (CoTC) element. We show that homopolymer A/T tract...

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Bibliographic Details
Published in:Nucleic acids research 2013-02, Vol.41 (3), p.1797-1806
Main Authors: White, Eleanor, Kamieniarz-Gdula, Kinga, Dye, Michael J, Proudfoot, Nick J
Format: Article
Language:English
Subjects:
AT Rich Sequence
Base Sequence
beta-Globins - genetics
HeLa Cells
Humans
Molecular Biology
Molecular Sequence Data
Mutation
Poly A - chemistry
Poly T - chemistry
RNA Cleavage
RNA Polymerase II - metabolism
Terminator Regions, Genetic
Transcription Termination, Genetic
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Summary:RNA Polymerase II (Pol II) termination is dependent on RNA processing signals as well as specific terminator elements located downstream of the poly(A) site. One of the two major terminator classes described so far is the Co-Transcriptional Cleavage (CoTC) element. We show that homopolymer A/T tracts within the human β-globin CoTC-mediated terminator element play a critical role in Pol II termination. These short A/T tracts, dispersed within seemingly random sequences, are strong terminator elements, and bioinformatics analysis confirms the presence of such sequences in 70% of the putative terminator regions (PTRs) genome-wide.
ISSN:0305-1048
1362-4962
DOI:10.1093/nar/gks1335