Three variations in rabbit angiographic stroke models

► Cerebral angiography techniques in the rabbit are described. ► Three embolic models of acute ischemic stroke are developed. ► Endpoints include infarct volume percent and neurological assessment score. ► Stroke models vary by embolus type and can be modified to fit research needs. Purpose: To deve...

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Bibliographic Details
Published in:Journal of neuroscience methods 2013-01, Vol.212 (2), p.322-328
Main Authors: Culp, William C., Woods, Sean D., Brown, Aliza T., Lowery, John D., Hennings, Leah J., Skinner, Robert D., Borrelli, Michael J., Roberson, Paula K.
Format: Article
Language:English
Subjects:
Angiography
Animal models
Animals
Cerebral Angiography - methods
Disease Models, Animal
Female
Focal cerebral ischemia
Intracranial Embolism - complications
Intracranial Embolism - diagnostic imaging
Male
Rabbit
Rabbits
Stroke
Stroke - diagnostic imaging
Stroke - etiology
Stroke - pathology
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Summary:► Cerebral angiography techniques in the rabbit are described. ► Three embolic models of acute ischemic stroke are developed. ► Endpoints include infarct volume percent and neurological assessment score. ► Stroke models vary by embolus type and can be modified to fit research needs. Purpose: To develop angiographic models of embolic stroke in the rabbit using pre-formed clot or microspheres to model clinical situations ranging from transient ischemic events to severe ischemic stroke. Materials and methods: New Zealand White rabbits (N=151) received angiographic access to the internal carotid artery (ICA) from a femoral approach. Variations of emboli type and quantity of emboli were tested by injection into the ICA. These included fresh clots (1.0-mm length, 3–6h), larger aged clots (4.0-mm length, 3 days), and 2 or 3 insoluble microspheres (700–900μm). Neurological assessment scores (NAS) were based on motor, sensory, balance, and reflex measures. Rabbits were euthanized at 4, 7, or 24h after embolization, and infarct volume was measured as a percent of total brain volume using 2,3,5-triphenyltetrazolium chloride (TTC). Results: Infarct volume percent at 24h after stroke was lower for rabbits embolized with fresh clot (0.45±0.14%), compared with aged clot (3.52±1.31%) and insoluble microspheres (3.39±1.04%). Overall NAS (including posterior vessel occlusions) were positively correlated to infarct volume percent measurements in the fresh clot (r=0.50), aged clot (r=0.65) and microsphere (r=0.62) models (p
ISSN:0165-0270
1872-678X
DOI:10.1016/j.jneumeth.2012.10.017