The manipulation of natural killer cells to target tumor sites using magnetic nanoparticles

Abstract The present work demonstrates that Cy5.5 conjugated Fe3 O4 /SiO2 core/shell nanoparticles could allow us to control movement of human natural killer cells (NK-92MI) by an external magnetic field. Required concentration of the nanoparticles for the cell manipulation is as low as ∼20 μg Fe/mL...

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Bibliographic Details
Published in:Biomaterials 2012-08, Vol.33 (22), p.5584-5592
Main Authors: Jang, Eue-Soon, Shin, June-Ho, Ren, Gang, Park, Mi-Jin, Cheng, Kai, Chen, Xiaoyuan, Wu, Joseph C, Sunwoo, John B, Cheng, Zhen
Format: Article
Language:English
Subjects:
Advanced Basic Science
Animals
Cell Line
Cell Movement - radiation effects
Dentistry
Fe3O4/SiO2 core/shell nanoparticles
Immunomagnetic Separation - methods
Killer Cells, Natural - radiation effects
Killer Cells, Natural - transplantation
Magnetic field guided cell control
Magnetic Fields
Magnetite Nanoparticles
Multifunctional nanoparticles
Natural killer cells
Neoplasms, Experimental - pathology
Neoplasms, Experimental - therapy
Rats
Treatment Outcome
Tumor killing activity
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Summary:Abstract The present work demonstrates that Cy5.5 conjugated Fe3 O4 /SiO2 core/shell nanoparticles could allow us to control movement of human natural killer cells (NK-92MI) by an external magnetic field. Required concentration of the nanoparticles for the cell manipulation is as low as ∼20 μg Fe/mL. However, the relative ratio of the nanoparticles loaded NK-92MI cells infiltrated into the target tumor site is enhanced by 17-fold by applying magnetic field and their killing activity is still maintained as same as the NK-92MI cells without the nanoparticles. This approach allows us to open alternative clinical treatment with reduced toxicity of the nanoparticles and enhanced infiltration of immunology to the target site.
ISSN:0142-9612
1878-5905
DOI:10.1016/j.biomaterials.2012.04.041