iSLIM: a comprehensive approach to mapping and characterizing gene regulatory networks

Mapping gene regulatory networks is a significant challenge in systems biology, yet only a few methods are currently capable of systems-level identification of transcription factors (TFs) that bind a specific regulatory element. We developed a microfluidic method for integrated systems-level interac...

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Bibliographic Details
Published in:Nucleic acids research 2013-02, Vol.41 (4), p.e52-e52
Main Authors: Rockel, Sylvie, Geertz, Marcel, Hens, Korneel, Deplancke, Bart, Maerkl, Sebastian J
Format: Article
Language:English
Subjects:
Animals
Base Sequence
Consensus Sequence
DNA - metabolism
Drosophila melanogaster - genetics
Gene Library
Gene Regulatory Networks
Methods Online
Microfluidic Analytical Techniques
Nucleotide Motifs
Position-Specific Scoring Matrices
Regulatory Elements, Transcriptional
Transcription Factors - analysis
Transcription Factors - genetics
Transcription Factors - metabolism
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Summary:Mapping gene regulatory networks is a significant challenge in systems biology, yet only a few methods are currently capable of systems-level identification of transcription factors (TFs) that bind a specific regulatory element. We developed a microfluidic method for integrated systems-level interaction mapping of TF-DNA interactions, generating and interrogating an array of 423 full-length Drosophila TFs. With integrated systems-level interaction mapping, it is now possible to rapidly and quantitatively map gene regulatory networks of higher eukaryotes.
ISSN:0305-1048
1362-4962
DOI:10.1093/nar/gks1323