A virus-like particle vaccine platform elicits heightened and hastened local lung mucosal antibody production after a single dose

• Published in: Vaccine 2012-05, Vol.30 (24), p.3653-3665
• Main Authors: Richert, Laura E, Servid, Amy E, Harmsen, Ann L, Rynda-Apple, Agnieszka, Han, Soo, Wiley, James A, Douglas, Trevor, Harmsen, Allen G
• Format: Article
• Language: English
• Subjects: Adjuvants; Administration, Intranasal; Allergy and Immunology; Animals; Antibodies, Viral - immunology; antibody formation; Antibody response; Antigens; bacteriophages; Biomedical research; blood serum; capsid; Capsids; Colleges & universities; disease control; Female; Genetic engineering; Germinal centers; Heat shock proteins; Helper cells; Homeostasis; Immune response; Immune system; Immunization; Immunoglobulin A; Immunoglobulin G; Influenza; Influenza Vaccines - administration & dosage; Influenza Vaccines - immunology; Influenza virus; Lung; lungs; Lymphocytes B; Male; Mice; Mice, Inbred BALB C; Mice, Inbred C57BL; Mucosa; Mucosal vaccine; Nanoparticle; Nanoparticles; Orthomyxoviridae; Ovalbumin; Ovalbumin - immunology; Phages; Prophylaxis; Respiratory Mucosa - immunology; small heat shock proteins; stoichiometry; Time Factors; Tissue-specific Immunity; Vaccination - methods; vaccine development; Vaccines; Vaccines, Conjugate - administration & dosage; Vaccines, Conjugate - immunology; Virus-like particle; virus-like particle vaccines; Virus-like particles
• ISSN: 0264-410X; 1873-2518
• DOI: 10.1016/j.vaccine.2012.03.035

Highlights ► We conjugated OVA to a sHsp nanoparticle, and show its utility for vaccination. ► The immune response to OVA–sHsp was quick and intense with a single i.n. dose. ► Delivery of OVA–sHsp resulted in the same immune response to OVA as sHsp itself. ► sHsp acted as an adjuvant in the lungs, and induced the production of mucosal IgA. ► Pre-treatment with sHsp provided protective ab and cellular responses to influenza.