Phage display of functional αβ single-chain T-cell receptor molecules specific for CD1b:Ac₂SGL complexes from Mycobacterium tuberculosis-infected cells

The development of molecules specific for M. tuberculosis-infected cells has important implications, as these tools may facilitate understanding of the mechanisms regulating host pathogen interactions in vivo. In addition, development of new tools capable to targeting M. tuberculosis-infected cells...

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Published in:BMC immunology 2013-02, Vol.14 Suppl 1 (S1), p.S2-S2, Article S2
Main Authors: Camacho, Frank, Huggett, Jim, Kim, Louise, Infante, Juan F, Lepore, Marco, Perez, Viviana, Sarmiento, María E, Rook, Graham, Acosta, Armando
Format: Article
Language:English
Subjects:
Antigens, Bacterial - immunology
Antigens, CD1 - immunology
Bacteriophage M13
Cell Line
Cell Surface Display Techniques
Genes, T-Cell Receptor alpha
Genes, T-Cell Receptor beta
Glycolipids - immunology
Humans
Lung - immunology
Lung - microbiology
Lymphocyte Activation
Mycobacterium tuberculosis - immunology
Proceedings
Receptors, Antigen, T-Cell, alpha-beta - genetics
Receptors, Antigen, T-Cell, alpha-beta - immunology
Recombinant Fusion Proteins - immunology
Tuberculosis - immunology
Viral Proteins
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Summary:The development of molecules specific for M. tuberculosis-infected cells has important implications, as these tools may facilitate understanding of the mechanisms regulating host pathogen interactions in vivo. In addition, development of new tools capable to targeting M. tuberculosis-infected cells may have potential applications to diagnosis, treatment, and prevention of tuberculosis (TB). Due to the lack of CD1b polymorphism, M. tuberculosis lipid-CD1b complexes could be considered as universal tuberculosis infection markers. The aim of the present study was to display on the PIII surface protein of m13 phage, a human αβ single-chain T-cell receptor molecule specific for CD1b:2-stearoyl-3-hydroxyphthioceranoyl-2´-sulfate-α-α´-D-trehalose (Ac₂SGL) which is a complex presented by human cells infected with M. tuberculosis. The results showed the pIII fusion particle was successfully displayed on the phage surface. The study of the recognition of the recombinant phage in ELISA and immunohistochemistry showed the recognition of CD1b:Ac₂SGL complexes and cells in human lung tissue from a tuberculosis patient respectively, suggesting the specific recognition of the lipid-CD1b complex.
ISSN:1471-2172
1471-2172
DOI:10.1186/1471-2172-14-S1-S2