Mapping and analysis of phosphorylation sites: a quick guide for cell biologists

A mechanistic understanding of signaling networks requires identification and analysis of phosphorylation sites. Mass spectrometry offers a rapid and highly sensitive approach to mapping phosphorylation sites. However, mass spectrometry has significant limitations that must be considered when planni...

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Bibliographic Details
Published in:Molecular biology of the cell 2013-03, Vol.24 (5), p.535-542
Main Authors: Dephoure, Noah, Gould, Kathleen L, Gygi, Steven P, Kellogg, Douglas R
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Amino Acids - chemistry
Chromosome Mapping
Databases, Protein
Peptide Fragments - chemistry
Phosphoproteins - chemistry
Phosphorylation
Signal Transduction
Tandem Mass Spectrometry - methods
Technical
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Summary:A mechanistic understanding of signaling networks requires identification and analysis of phosphorylation sites. Mass spectrometry offers a rapid and highly sensitive approach to mapping phosphorylation sites. However, mass spectrometry has significant limitations that must be considered when planning to carry out phosphorylation-site mapping. Here we provide an overview of key information that should be taken into consideration before beginning phosphorylation-site analysis, as well as a step-by-step guide for carrying out successful experiments.
ISSN:1059-1524
1939-4586
DOI:10.1091/mbc.e12-09-0677