Enhancement of carotenoid biosynthesis in the green microalga Dunaliella salina with light-emitting diodes and adaptive laboratory evolution

• Published in: Applied microbiology and biotechnology 2013-03, Vol.97 (6), p.2395-2403
• Main Authors: Fu, Weiqi, Guðmundsson, Ólafur, Paglia, Giuseppe, Herjólfsson, Gísli, Andrésson, Ólafur S., Palsson, Bernhard Ø., Brynjólfsson, Sigurður
• Format: Article
• Language: English
• Subjects: Accumulation; Algae; Antennas; Biological Evolution; Biomass; Biomedical and Life Sciences; Biosynthesis; Biotechnological Products and Process Engineering; Biotechnology; Biotechnology - methods; blue light; Carotenoids; Carotenoids - biosynthesis; Chlamydomonas reinhardtii; Chlorophyll; Chromatography, Liquid; Dunaliella salina; Evolution; Experiments; Flux; Gene expression; Green algae; growth & development; Laboratories; Life Sciences; Light; Light emitting diodes; Light quality; Lutein; markets; Mass Spectrometry; Metabolism; methods; Microalgae; Microbial Genetics and Genomics; Microbiology; Photons; Physiological aspects; Plant biology; radiation effects; red light; Salina; Spectrophotometry, Ultraviolet; Spectrum allocation; Studies; Volvocida; Volvocida - growth & development; Volvocida - metabolism; Volvocida - radiation effects
• ISSN: 0175-7598; 1432-0614
• DOI: 10.1007/s00253-012-4502-5

There is a particularly high interest to derive carotenoids such as β-carotene and lutein from higher plants and algae for the global market. It is well known that β-carotene can be overproduced in the green microalga Dunaliella salina in response to stressful light conditions. However, little is known about the effects of light quality on carotenoid metabolism, e.g., narrow spectrum red light. In this study, we present UPLC-UV-MS data from D. salina consistent with the pathway proposed for carotenoid metabolism in the green microalga Chlamydomonas reinhardtii . We have studied the effect of red light-emitting diode (LED) lighting on growth rate and biomass yield and identified the optimal photon flux for D. salina growth. We found that the major carotenoids changed in parallel to the chlorophyll b content and that red light photon stress alone at high level was not capable of upregulating carotenoid accumulation presumably due to serious photodamage. We have found that combining red LED (75 %) with blue LED (25 %) allowed growth at a higher total photon flux. Additional blue light instead of red light led to increased β-carotene and lutein accumulation, and the application of long-term iterative stress (adaptive laboratory evolution) yielded strains of D. salina with increased accumulation of carotenoids under combined blue and red light.