Display of the Viral Epitopes on Lactococcus lactis: A Model for Food Grade Vaccine against EV71

In this study, we have developed a system for display of antigens of Enterovirus type 71 (EV71) on the cell surface of L. lactis. The viral capsid protein (VP1) gene from a local viral isolate was utilized as the candidate vaccine for the development of oral live vaccines against EV71 using L. lacti...

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Bibliographic Details
Published in:Biotechnology Research International 2013-01, Vol.2013 (2013), p.48-56
Main Authors: Varma, Nadimpalli Ravi S., Toosa, Haryanti, Foo, Hooi Ling, Alitheen, Noorjahan Banu Mohamed, Shamsudin, Mariana Nor, Arbab, Ali S., Yusoff, Khatijah, Abdul Rahim, Raha
Format: Article
Language:English
Subjects:
Analysis
Antigenic determinants
Capsid protein
Enterovirus
Lactococcus
Product development
Viral vaccines
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Summary:In this study, we have developed a system for display of antigens of Enterovirus type 71 (EV71) on the cell surface of L. lactis. The viral capsid protein (VP1) gene from a local viral isolate was utilized as the candidate vaccine for the development of oral live vaccines against EV71 using L. lactis as a carrier. We expressed fusion proteins in E. coli and purified fusion proteins were incubated with L. lactis. We confirmed that mice orally fed with L. lactis displaying these fusion proteins on its surface were able to mount an immune response against the epitopes of EV71. This is the first example of an EV71 antigen displayed on the surface of a food grade organism and opens a new perspective for alternative vaccine strategies against the EV71. We believe that the method of protein docking utilized in this study will allow for more flexible presentations of short peptides and proteins on the surface of L. lactis to be useful as a delivery vehicle.
ISSN:2090-3138
2090-3146
2090-3146
DOI:10.1155/2013/431315