The Influence of Recombinant Human Erythropoietin on Apoptosis and Cytokine Production of CD4+ lymphocytes from Hemodialyzed Patients

Recombinant human erythropoietin (rhEPO) treatment of hemodialyzed (HD) patients normalizes the altered phenotype of CD4 + lymphocytes and restores the balance of Th1/Th2 cytokines. We decided to test how the presence of rhEPO in cell culture modulates cytokine production of CD4 + lymphocytes in HD...

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Bibliographic Details
Published in:Journal of clinical immunology 2013-04, Vol.33 (3), p.661-665
Main Authors: Lisowska, Katarzyna A., Dębska-Ślizień, Alicja, Jasiulewicz, Aleksandra, Daca, Agnieszka, Bryl, Ewa, Witkowski, Jacek M.
Format: Article
Language:English
Subjects:
Aged
Apoptosis - drug effects
Biomedical and Life Sciences
Biomedicine
Brief Communication
CD4-Positive T-Lymphocytes - immunology
CD4-Positive T-Lymphocytes - metabolism
Cells, Cultured
Cytokines - biosynthesis
Erythropoietin - pharmacology
Female
Humans
Immunology
Infectious Diseases
Internal Medicine
Male
Medical Microbiology
Middle Aged
Recombinant Proteins
Renal Dialysis
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Summary:Recombinant human erythropoietin (rhEPO) treatment of hemodialyzed (HD) patients normalizes the altered phenotype of CD4 + lymphocytes and restores the balance of Th1/Th2 cytokines. We decided to test how the presence of rhEPO in cell culture modulates cytokine production of CD4 + lymphocytes in HD patients with stable hemoglobin level and expression of activation antigens of stimulated CD4 + lymphocytes similar to those observed in healthy individuals. We also tested whether the presence of rhEPO in cell culture protects stimulated CD4 + lymphocytes of HD patients from apoptosis. Peripheral blood mononuclear cells (PBMC) of HD patients were stimulated with an immobilized anti-CD3 antibody with or without addition of rhEPO. The percentage of apoptotic CD4 + lymphocytes and the level of Th1/Th2 cytokines in culture supernatants were measured with flow cytometry. HD patients showed a decrease in the percentage of apoptotic CD4 + cells after stimulation with the anti-CD3 antibody combined with rhEPO. The level of IFN-γ and IL-10 was increased while the level of TNF-α was decreased in the presence of rhEPO in cell culture from HD patients. These results confirm the role of rhEPO signaling in T lymphocytes of HD patients.
ISSN:0271-9142
1573-2592
DOI:10.1007/s10875-012-9835-4