Neutrophils alter epithelial response to Porphyromonas gingivalis in a gingival crevice model

Summary A gingival crevice model (epithelial cell–Porphyromonas gingivalis–neutrophil) was established and used to profile gingipain, matrix metalloproteinase (MMP), MMP mediators [neutrophil gelatinase‐associated lipocalin (NGAL) and tissue inhibitor of metalloproteinases 1 (TIMP‐1)] and cytokine n...

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Bibliographic Details
Published in:Molecular oral microbiology 2013-04, Vol.28 (2), p.102-113
Main Authors: Bondy-Carey, J.L., Galicia, J., Bagaitkar, J., Potempa, J.S., Potempa, B., Kinane, D.F., Veillard, F., Scott, D.A.
Format: Article
Language:English
Subjects:
Acute-Phase Proteins - analysis
Adhesins, Bacterial - analysis
Adhesins, Bacterial - pharmacology
Cell Culture Techniques
Cells, Cultured
Cysteine Endopeptidases - analysis
Cysteine Endopeptidases - pharmacology
cytokines
Cytokines - analysis
Disease Progression
Disease Susceptibility
epithelial cells
Epithelial Cells - enzymology
Epithelial Cells - physiology
Gingipain Cysteine Endopeptidases
Gingiva - immunology
Gingiva - microbiology
Humans
Inflammation Mediators - analysis
Interleukin-1beta - analysis
Interleukin-8 - analysis
Lipocalin-2
Lipocalins - analysis
Matrix Metalloproteinase 8 - analysis
Matrix Metalloproteinase 9 - analysis
Matrix Metalloproteinase 9 - drug effects
matrix metalloproteinases
Microbial Viability
neutrophils
Neutrophils - enzymology
Neutrophils - physiology
Nicotiana
Nicotine - pharmacology
periodontitis
Porphyromonas gingivalis
Porphyromonas gingivalis - immunology
Porphyromonas gingivalis - physiology
Proto-Oncogene Proteins - analysis
Smoke
Tissue Inhibitor of Metalloproteinase-1 - analysis
Tissue Inhibitor of Metalloproteinase-1 - drug effects
tobacco smoke
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Summary:Summary A gingival crevice model (epithelial cell–Porphyromonas gingivalis–neutrophil) was established and used to profile gingipain, matrix metalloproteinase (MMP), MMP mediators [neutrophil gelatinase‐associated lipocalin (NGAL) and tissue inhibitor of metalloproteinases 1 (TIMP‐1)] and cytokine networks. Smoking is the primary environmental risk factor for periodontitis. Therefore, the influence of cigarette smoke extract (CSE) was also monitored in the same model. Porphyromonas gingivalis alone induced low levels of interleukin‐1β and interleukin‐8 from epithelial cells, but high levels of both cytokines were produced on the addition of neutrophils. Exposure to CSE (100 and 1000 ng ml−1 nicotine equivalency) significantly compromised P. gingivalis‐induced cytokine secretion (both P 
ISSN:2041-1006
2041-1014
DOI:10.1111/omi.12008