Breaking the Diffraction Barrier Using Fluorescence Emission Difference Microscopy

We propose a novel physical mechanism for breaking the diffraction barrier in the far field. Termed fluorescence emission difference microscopy (FED), our approach is based on the intensity difference between two differently acquired images. When fluorescence saturation is applied, the resolving abi...

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Bibliographic Details
Published in:Scientific reports 2013-03, Vol.3 (1), p.1441-1441, Article 1441
Main Authors: Kuang, Cuifang, Li, Shuai, Liu, Wei, Hao, Xiang, Gu, Zhaotai, Wang, Yifan, Ge, Jianhong, Li, Haifeng, Liu, Xu
Format: Article
Language:English
Subjects:
639/624/1107/328/2238
639/624/1107/510
639/624/1111/55
639/624/400/385
Diffraction
Humanities and Social Sciences
Microscopy
multidisciplinary
Nanoparticles
Science
Spatial discrimination
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Summary:We propose a novel physical mechanism for breaking the diffraction barrier in the far field. Termed fluorescence emission difference microscopy (FED), our approach is based on the intensity difference between two differently acquired images. When fluorescence saturation is applied, the resolving ability of FED can be further enhanced. A detailed theoretical analysis and a series of simulation tests are performed. The validity of FED in practical use is demonstrated by experiments on fluorescent nanoparticles and biological cells in which a spatial resolution of
ISSN:2045-2322
2045-2322
DOI:10.1038/srep01441