Generation of conditional knockout alleles for PRL-3

Phosphatase of regenerating liver-3 （PRL-3） is a member of the protein tyrosine phosphatase （PTP） superfamily and is highly expressed in cancer metastases. For better understanding of the role of PRL-3 in tumor metastasis, we applied a rapid and efficient method for generating PRL-3 floxed mice and...

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Bibliographic Details
Published in:Journal of biomedical research 2011-11, Vol.25 (6), p.438-443
Main Authors: Yan, Hong, Kong, Dong, Ge, Xiaomei, Gao, Xiang, Han, Xiao
Format: Article
Language:English
Subjects:
conditional knockout alleles
Cre recombinase
PRL-3
Research Paper
RT-PCR分析
Southern杂交
tumor metastasis
发电
基因敲除
等位基因
肿瘤转移
蛋白酪氨酸磷酸酶
表型特征
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Summary:Phosphatase of regenerating liver-3 （PRL-3） is a member of the protein tyrosine phosphatase （PTP） superfamily and is highly expressed in cancer metastases. For better understanding of the role of PRL-3 in tumor metastasis, we applied a rapid and efficient method for generating PRL-3 floxed mice and investigated its phenotypes. A BAC retrieval strategy was applied to construct the PRL-3 conditional gene-targeting vector. Exon 4 was selected for deletion to generate a nonfunctional prematurely terminated short peptide as it will cause a frame-shift mutation. Conditional knockout PRL-3 mice were generated by using the Cre-loxP system and were validated by Southern blot and RT-PCR analysis. Further analysis revealed the phenotype characteristics of PRL-3 knockout mice and wildtype mice. In this study, we successfully constructed the PRL-3 conditional knockout mice, which will be helpful to clarify the roles of PRL-3 and the mechanisms in tumor metastasis.
ISSN:1674-8301
DOI:10.1016/S1674-8301(11)60058-4