New image colocalization coefficient for fluorescence microscopy to quantify (bio‐)molecular interactions

Summary The spatial relationship, or degree of colocalization, between two or more types of molecules in live cells is commonly detected using fluorescence microscopy. This spatial distribution can be used to estimate the interaction between fluorescently labelled molecules. These interactions are u...

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Bibliographic Details
Published in:Journal of microscopy (Oxford) 2013-03, Vol.249 (3), p.184-194
Main Authors: HERCE, H.D., CASAS‐DELUCCHI, C.S., CARDOSO, M.C.
Format: Article
Language:English
Subjects:
Biomolecules
Coefficients
Colocalization
Confocal Microscopy
Fluorescence
Fluorescent Microscopy
Free energy
High resolution microscopy
Image Processing, Computer-Assisted - methods
Mathematical analysis
Microscopy
Microscopy, Confocal - methods
Microscopy, Fluorescence - methods
Molecular Imaging - methods
Molecular Interactions
Original
Proteins
Spatial distribution
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Description
Summary:Summary The spatial relationship, or degree of colocalization, between two or more types of molecules in live cells is commonly detected using fluorescence microscopy. This spatial distribution can be used to estimate the interaction between fluorescently labelled molecules. These interactions are usually quantified by analysing the correlation and/or the overlap between images, using the Pearson's and Manders’ coefficients, respectively. However, the correlation and overlap coefficients are parameters not designed to quantify molecular interactions. Here we propose a new colocalization coefficient specifically designed to quantify the interactions between molecules. In well‐defined thermodynamic ensembles, this coefficient can in principle be used to calculate relevant statistical thermodynamic quantities such as binding free energies.
ISSN:0022-2720
1365-2818
DOI:10.1111/jmi.12008