Sweet Chestnut (Castanea sativa Mill.) Bark Extract : Cardiovascular Activity and Myocyte Protection against Oxidative Damage

This work was aimed at evaluating the cardioprotective effects of Castanea sativa Mill. (CSM) bark extract characterized in its phenolic composition by HPLC-DAD-MS analysis. The study was performed using primary cultures of neonatal rat cardiomyocytes to investigate the antioxidant and cytoprotectiv...

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Bibliographic Details
Published in:Oxidative medicine and cellular longevity 2013-01, Vol.2013 (2013), p.1-10
Main Authors: Chiarini, Alberto, Micucci, Matteo, Malaguti, Marco, Budriesi, Roberta, Ioan, Pierfranco, Lenzi, Monia, Fimognari, Carmela, Gallina Toschi, Tullia, Comandini, Patrizia, Hrelia, Silvana
Format: Article
Language:English
Subjects:
Animals
Animals, Newborn
Atrial Function - drug effects
Cardiotonic Agents - chemistry
Cardiotonic Agents - pharmacology
Cell Survival - drug effects
Cells, Cultured
Fagaceae - chemistry
Guinea Pigs
In Vitro Techniques
Myocytes, Cardiac - drug effects
Myocytes, Cardiac - metabolism
Oxidative Stress - drug effects
Papillary Muscles - drug effects
Papillary Muscles - physiology
Plant Bark - chemistry
Plant Extracts - chemistry
Plant Extracts - pharmacology
Rats
Reactive Oxygen Species - metabolism
Receptors, Adrenergic - metabolism
Receptors, Cholinergic - metabolism
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Summary:This work was aimed at evaluating the cardioprotective effects of Castanea sativa Mill. (CSM) bark extract characterized in its phenolic composition by HPLC-DAD-MS analysis. The study was performed using primary cultures of neonatal rat cardiomyocytes to investigate the antioxidant and cytoprotective effects of CSM bark extract and isolated guinea pig left and right atria, left papillary muscle, and aorta to evaluate its direct effect on cholinergic and adrenergic response. In cultured cardiomyocytes the CSM bark extract reduced intracellular reactive oxygen species formation and improved cell viability following oxidative stress in dose-dependent manner. Moreover, the extract decreased the contraction induced by noradrenaline (1 μM) in guinea pig aortic strips and induced transient negative chronotropic and positive inotropic effects without involvement of cholinergic or adrenergic receptors in the guinea pig atria. Our results indicate that CSM bark extract exhibits antioxidant activity and might induce cardioprotective effect.
ISSN:1942-0900
1942-0994
DOI:10.1155/2013/471790