Tetrandrine Inhibits the Wnt/β-Catenin Signalling Pathway and Alleviates Osteoarthritis : An In Vitro and In Vivo Study

There is currently no effective drug treatment for the early phase of osteoarthritis (OA), one of the most common senile diseases. The goal of this study was to investigate the protective effect of the tetrandrine (Tet) on OA, in vitro and in vivo. In an in vitro experiment, quantitative real-time p...

Full description

Saved in:
Bibliographic Details
Published in:Evidence-based complementary and alternative medicine 2013-01, Vol.2013 (2013), p.1-8
Main Authors: Li, Weijun, Zhou, Xindie, Jiang, Lifeng, Bao, Jiapeng, Tao, Lijiang, Li, Jin, Wu, Lidong
Format: Article
Language:English
Subjects:
Anterior cruciate ligament
Arthritis
Articular
Cartilage (articular)
Cartilage diseases
Chondrocytes
Clinical medicine
Colorectal cancer
Cytokines
Disease
Enzymes
Gene expression
IL-1β
Joint and ligament injuries
Joint surgery
Matrix metalloproteinase
Metalloproteinase
Nonsteroidal anti-inflammatory drugs
Osteoarthritis
Pathogenesis
Polymerase chain reaction
Rheumatology
Signal transduction
Tetrandrine
Tissue inhibitor of metalloproteinase 1
Western blotting
Wnt protein
β-Catenin
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:There is currently no effective drug treatment for the early phase of osteoarthritis (OA), one of the most common senile diseases. The goal of this study was to investigate the protective effect of the tetrandrine (Tet) on OA, in vitro and in vivo. In an in vitro experiment, quantitative real-time polymerase chain reaction (qRT-PCR) was used to investigate changes in gene expression upon the addition of Tet in chondrocytes processed with IL-1β; changes in protein profiles were assessed by Western blotting. In vivo, to determine whether Tet has the protective effects on articular cartilage, a rabbit anterior cruciate ligament transaction model of OA was established. Expression of matrix metalloproteinase and β-catenin genes increased significantly, while that of tissue inhibitor of metalloproteinase-1 decreased significantly in the OA group both in vivo and in chondrocytes. However, the changes of expression were reversed by Tet, and there was less cartilage degradation in vivo compared with the OA group, as assessed by histological and macroscopic observations. Thus, Tet may play a useful role in the treatment of OA through the Wnt/β-catenin signalling pathway and has potential for the treatment of OA.
ISSN:1741-427X
1741-4288
DOI:10.1155/2013/809579