Tyrosinase as a multifunctional reporter gene for Photoacoustic/MRI/PET triple modality molecular imaging

Development of reporter genes for multimodality molecular imaging is highly important. In contrast to the conventional strategies which have focused on fusing several reporter genes together to serve as multimodal reporters, human tyrosinase (TYR) – the key enzyme in melanin production – was evaluat...

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Bibliographic Details
Published in:Scientific reports 2013-03, Vol.3 (1), p.1490-1490, Article 1490
Main Authors: Qin, Chunxia, Cheng, Kai, Chen, Kai, Hu, Xiang, Liu, Yang, Lan, Xiaoli, Zhang, Yongxue, Liu, Hongguang, Xu, Yingding, Bu, Lihong, Su, Xinhui, Zhu, Xiaohua, Meng, Shuxian, Cheng, Zhen
Format: Article
Language:English
Subjects:
631/1647/245/2160
631/67/2321
639/638/45/56
692/308/575
Animals
Breast cancer
Cell Line, Tumor
Disease Models, Animal
Female
Genes
Genes, Reporter
Heterografts
Humanities and Social Sciences
Humans
Magnetic resonance imaging
Magnetic Resonance Imaging - methods
Medical imaging
Melanin
Melanins - biosynthesis
Mice
Molecular Imaging - methods
Monophenol Monooxygenase - genetics
Monophenol Monooxygenase - metabolism
multidisciplinary
Neoplasms - diagnosis
Neoplasms - genetics
Neoplasms - metabolism
NMR
Nuclear magnetic resonance
Positron emission tomography
Positron-Emission Tomography - methods
Radiopharmaceuticals
Reporter gene
Science
Tomography
Tumors
TYR gene
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Summary:Development of reporter genes for multimodality molecular imaging is highly important. In contrast to the conventional strategies which have focused on fusing several reporter genes together to serve as multimodal reporters, human tyrosinase (TYR) – the key enzyme in melanin production – was evaluated in this study as a stand-alone reporter gene for in vitro and in vivo photoacoustic imaging (PAI), magnetic resonance imaging (MRI) and positron emission tomography (PET). Human breast cancer cells MCF-7 transfected with a plasmid that encodes TYR (named as MCF-7-TYR) and non-transfected MCF-7 cells were used as positive and negative controls, respectively. Melanin targeted N-(2-(diethylamino)ethyl)- 18 F-5-fluoropicolinamide was used as a PET reporter probe. In vivo PAI/MRI/PET imaging studies showed that MCF-7-TYR tumors achieved significant higher signals and tumor-to-background contrasts than those of MCF-7 tumor. Our study demonstrates that TYR gene can be utilized as a multifunctional reporter gene for PAI/MRI/PET both in vitro and in vivo .
ISSN:2045-2322
2045-2322
DOI:10.1038/srep01490