Inflammatory Regulation Effect and Action Mechanism of Anti-Inflammatory Effective Parts of Housefly (Musca domestica) Larvae on Atherosclerosis

The protein-enriched extracts of housefly larvae were segregated by gel-filtration chromatography (GFC) and then anti-inflammatory activity screening in RAW264.7 (induced by LPS) was carried out. After acquire the anti-inflammatory effective parts, its anti-atherosclerotic properties in vivo were th...

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Bibliographic Details
Published in:Evidence-based complementary and alternative medicine 2013-01, Vol.2013 (2013), p.1-10
Main Authors: Chu, Fu Jiang, Jin, Xiao Bao, Xu, Yin Ye, Ma, Yan, Li, Xiao Bo, Lu, Xue Mei, Liu, Wen Bin, Zhu, Jia Yong
Format: Article
Language:English
Subjects:
Anti-inflammatory agents
Arteriosclerosis
Atherosclerosis
Cytoplasm
Gel-filtration chromatography
High density lipoprotein
Humidity
Immunofluorescence
Immunology
Inflammation
Insects
Kinases
Laboratory animals
Larvae
Lesions
Lipopolysaccharides
Low molecular weights
Metabolism
Microscopy
Morphology
Musca domestica
NF-κB protein
Proteins
Tumor necrosis factor-TNF
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Summary:The protein-enriched extracts of housefly larvae were segregated by gel-filtration chromatography (GFC) and then anti-inflammatory activity screening in RAW264.7 (induced by LPS) was carried out. After acquire the anti-inflammatory effective parts, its anti-atherosclerotic properties in vivo were then evaluated. Results showed that the anti-inflammatory effective parts of housefly larvae were low-molecular-weight parts. After treated with the effective parts oral gavaged for 4 weeks, the atherosclerotic lesions of the mouse were significantly decreased. The inflammatory and lipid parameters were also reduced (except HDL which was increased). Western blot analysis demonstrated that the effective parts exerted potent inhibitory effect on expression of p65 in nucleus and cytoplasm. The results of immunofluorescence microscopy analysis also showed that the expressions of p65 both in cytoplasm and nucleus were significantly reduced. The hypothesis that the anti-inflammatory effective parts of housefly larvae possessed anti-atherosclerosis activity in mouse and the possible mechanism could be associated with the inhibition of expression and nuclear transfer of NF-κB p65 could be derived.
ISSN:1741-427X
1741-4288
DOI:10.1155/2013/340267