Towards a universal vaccine for avian influenza: Protective efficacy of modified Vaccinia virus Ankara and Adenovirus vaccines expressing conserved influenza antigens in chickens challenged with low pathogenic avian influenza virus

• Published in: Vaccine 2013-01, Vol.31 (4), p.670-675
• Main Authors: Boyd, Amy C, Ruiz-Hernandez, Raul, Peroval, Marylene Y, Carson, Connor, Balkissoon, Devanand, Staines, Karen, Turner, Alison V, Hill, Adrian V.S, Gilbert, Sarah C, Butter, Colin
• Format: Article
• Language: English
• Subjects: Adenoviridae - genetics; Adenovirus; Age; Allergy and Immunology; Animals; antigenic variation; Applied microbiology; avian influenza; Avian influenza virus; Biological and medical sciences; cell-mediated immunity; Chicken; Chickens; Enzyme-linked immunosorbent assay; Epitopes; Fowl plague; Fundamental and applied biological sciences. Psychology; Genetic Vectors; Immune response; Immunization, Secondary; Immunogenicity; inbred lines; Inbreeding; Infection; Influenza; Influenza A virus; Influenza A Virus, H7N7 Subtype - genetics; Influenza A Virus, H7N7 Subtype - immunology; Influenza A Virus, H7N7 Subtype - pathogenicity; Influenza in Birds - immunology; Influenza in Birds - prevention & control; Influenza in Birds - virology; Influenza Vaccines - administration & dosage; Influenza Vaccines - adverse effects; Influenza Vaccines - genetics; Influenza Vaccines - immunology; interferon-gamma; Interferon-gamma - metabolism; LPAI; Lymphocytes T; matrix protein; Microbiology; Miscellaneous; MVA; Nucleocapsid Proteins; Nucleoproteins; Plaque assay; Poultry; Recombinant Proteins - genetics; Recombinant Proteins - immunology; Recombinant Proteins - metabolism; RNA-Binding Proteins - genetics; RNA-Binding Proteins - immunology; RNA-Binding Proteins - metabolism; secondary immunization; Studies; surface proteins; T-lymphocytes; T-Lymphocytes - immunology; Vaccination; Vaccine; vaccine development; Vaccines; Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies (general aspects); Vaccinia virus; Vaccinia virus - genetics; Vectors; Viral Core Proteins - genetics; Viral Core Proteins - immunology; Viral Core Proteins - metabolism; Viral Matrix Proteins - genetics; Viral Matrix Proteins - immunology; Viral Matrix Proteins - metabolism; Virology; Virus Shedding; viruses
• ISSN: 0264-410X; 1873-2518
• DOI: 10.1016/j.vaccine.2012.11.047

Highlights ► Current influenza vaccines do not generate heterologous protection. ► Targeting internal influenza antigens may confer cross protection. ► We tested Adenovirus and MVA vectored NP and M1 in chickens. ► Heterologous prime-boost resulted in earlier cessation of viral shedding.