Thrombin induces an inflammatory phenotype in a human brain endothelial cell line

Abstract In this study, we utilized the human brain endothelial cell line, hCMEC/D3, to determine the effects of the coagulation factor, thrombin, on the human blood–brain barrier (BBB). We show that thrombin increased the mRNA and cell surface levels of ICAM-1 and VCAM-1 in hCMEC/D3 cells. Thrombin...

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Bibliographic Details
Published in:Journal of neuroimmunology 2012-04, Vol.245 (1), p.48-55
Main Authors: Alabanza, Leah M, Bynoe, Margaret S
Format: Article
Language:English
Subjects:
Allergy and Immunology
Blood-Brain Barrier - immunology
Blood-Brain Barrier - metabolism
Blood-Brain Barrier - pathology
Blood–brain barrier
Cell Adhesion - drug effects
Cell Adhesion - immunology
Cell Line
Endothelial cells
Endothelial Cells - immunology
Endothelial Cells - metabolism
Endothelial Cells - pathology
hCMEC/D3
Humans
Inflammation - genetics
Inflammation - immunology
Inflammation - metabolism
Inflammation Mediators - toxicity
Neurology
Phenotype
Thrombin
Thrombin - toxicity
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Summary:Abstract In this study, we utilized the human brain endothelial cell line, hCMEC/D3, to determine the effects of the coagulation factor, thrombin, on the human blood–brain barrier (BBB). We show that thrombin increased the mRNA and cell surface levels of ICAM-1 and VCAM-1 in hCMEC/D3 cells. Thrombin similarly upregulated several chemokines implicated in human neurological conditions. Additionally, the paracellular permeability of the human BBB in vitro was also increased following thrombin treatment. Overall, this study demonstrates that thrombin can effectively induce an inflamed phenotype in an in vitro human BBB.
ISSN:0165-5728
1872-8421
DOI:10.1016/j.jneuroim.2012.02.004