In vivo antioxidant and hepatoprotective activity of methanolic extracts of Daucus carota seeds in experimental animals

• Published in: Asian Pacific journal of tropical biomedicine 2012-05, Vol.2 (5), p.385-388
• Main Authors: Singh, Kamlesh, Singh, Nisha, Chandy, Anish, Manigauha, Ashish
• Format: Article
• Language: English
• Subjects: Alanine Transaminase; Animals; Antioxidant; Antioxidants - pharmacology; Aspartate Aminotransferases; Biochemical; Biochemical parameters; carota; Chemical and Drug Induced Liver Injury - drug therapy; Chemical and Drug Induced Liver Injury - pathology; Daucus; Daucus carota - chemistry; Daucus carota seeds extract; Disease Models, Animal; extract; Glutathione; Glutathione Peroxidase; Hepatoprotection; Lipid Peroxidation; Liver - drug effects; Liver - pathology; Methanol; Original; Oxidative Stress - drug effects; parameters; Phytotherapy - methods; Plant Extracts - pharmacology; Rats; seeds; Seeds - chemistry; Silymarin; Superoxide Dismutase; Thioacetamide
• ISSN: 2221-1691; 2588-9222
• DOI: 10.1016/S2221-1691(12)60061-6

Objective:To assess the In vivo anlioxid Fanl and hepaloproleclive activity of metlianolic exlracl of Daucus carota(D.carota) seeds in experimental animals.Methods:Methanolic extracts of D.carota seeds is used for hepatoproleclion assessment.Oxidative stress were induced in rats by thioacetamide 100 nig/kg s.c.in four groups of rats(two test,standard and toxic control). Two test groups received D.carota seeds extract[DCSE) at doses of 200 mg/kg and 400 mg/kg. Standard group received silymarin(25 mg/kg) and toxic control received only thioacetamide. Control group received only vehicle.On the 8th day animals were sacrificed and liver enzyme like serum glutamic pyruvic transaminase(SGPT),serum glutamic-oxaloacetic transaminase(SCOT) and alkaline phosphatase(ALP)were estimated in blood serum and antioxidant enzyme like superoxide disnuituse(SOD),cululase(CAT),glutathione reductase(CKD),glutathione peroxidase(GPX),glutalhione-S-transferase(GST)and lipid peroxidation(LPO)were estimated in liver homogcnatc.Results:A significant decrease in SGPT,SCOT and ALP levels was observed in all drug treated groups as compared to thioacetamide group(P<0.001) and in case of antioxidant enzyme a significant(P<0.001) increase in SOD.CAT,GRD,GPX and GST was observed in all dmg treated groups as compared with thioacetamide group.But in case of LPO a significant(P <0.001) reduction was observed as compared to toxic control group.Conclusions:DCSE has contributed lo the reduction of oxidative stress and the protection of liver in experimental rals.