Chemically specific imaging of cryptosporidium oocysts using coherent anti-Stokes Raman scattering (CARS) microscopy

We demonstrate the application of coherent anti-Stokes Raman scattering microscopy for the rapid, label-free chemical imaging of waterborne pathogens. Chemically selective images of cryptosporidium were acquired in just a few seconds using coherent anti-Stokes Raman scattering microscopy, demonstrat...

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Bibliographic Details
Published in:Journal of microscopy (Oxford) 2009-02, Vol.233 (2), p.244-250
Main Authors: MURUGKAR, S, EVANS, C.L, XIE, X.S, ANIS, H
Format: Article
Language:English
Subjects:
Animals
Cattle
Coherent anti-Stokes Raman scattering
Cryptosporidium
Cryptosporidium - growth & development
Cryptosporidium - isolation & purification
Cryptosporidium - ultrastructure
cryptosporidium oocyst
Equipment Design
microscopy
Microscopy, Confocal - instrumentation
Microscopy, Confocal - methods
Oocysts - ultrastructure
Spectrum Analysis, Raman - instrumentation
Spectrum Analysis, Raman - methods
Water - parasitology
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Description
Summary:We demonstrate the application of coherent anti-Stokes Raman scattering microscopy for the rapid, label-free chemical imaging of waterborne pathogens. Chemically selective images of cryptosporidium were acquired in just a few seconds using coherent anti-Stokes Raman scattering microscopy, demonstrating its capability for the rapid detection of cryptosporidium at the single oocyst level. We discuss the applicability of such a technique in a near-real time automated water testing system.
ISSN:0022-2720
1365-2818
DOI:10.1111/j.1365-2818.2009.03114.x