Detection of Streptococcus equi subspecies equi using a triplex qPCR assay

Genome sequencing data for Streptococcus equi subspecies equi and zooepidemicus were used to develop a novel diagnostic triplex quantitative PCR (qPCR) assay targeting two genes specific to S. equi (eqbE and SEQ2190) and a unique 100 base pair control DNA sequence (SZIC) inserted into the SZO07770 p...

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Bibliographic Details
Published in:The veterinary journal (1997) 2013-03, Vol.195 (3), p.300-304
Main Authors: Webb, Katy, Barker, Colin, Harrison, Tihana, Heather, Zoe, Steward, Karen F., Robinson, Carl, Newton, J. Richard, Waller, Andrew S.
Format: Article
Language:English
Subjects:
bacteria
DNA
DNA, Bacterial - genetics
Equine
Genome, Bacterial
nucleotide sequences
Polymerase Chain Reaction - methods
pseudogenes
quantitative polymerase chain reaction
Reproducibility of Results
Sensitivity and Specificity
sequence analysis
Strangles
Streptococcus equi
Streptococcus equi - classification
Streptococcus equi - genetics
Streptococcus equi - isolation & purification
Triplex quantitative PCR
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Summary:Genome sequencing data for Streptococcus equi subspecies equi and zooepidemicus were used to develop a novel diagnostic triplex quantitative PCR (qPCR) assay targeting two genes specific to S. equi (eqbE and SEQ2190) and a unique 100 base pair control DNA sequence (SZIC) inserted into the SZO07770 pseudogene of S. zooepidemicus strain H70. This triplex strangles qPCR assay can provide results within 2h of sample receipt, has an overall sensitivity of 93.9% and specificity of 96.6% relative to the eqbE singlex assay and detects S. equi at levels below the threshold of the culture assay, even in the presence of contaminating bacteria.
ISSN:1090-0233
1532-2971
DOI:10.1016/j.tvjl.2012.07.007