Comparison of line probe assay with liquid culture for rapid detection of multi-drug resistance in Mycobacterium tuberculosis

Conventional drug susceptibility using solid medium such as Lowenstein - Jensen (L-J) is time consuming whereas liquid medium based methods such as Mycobacterial Growth Indicator Tube (MGIT) System 960 (BD Diagnostics, USA) are sensitive and faster but involve prohibitive expenditure [3] . Commercia...

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Bibliographic Details
Published in:Indian journal of medical research (New Delhi, India : 1994) India : 1994), 2012-12, Vol.136 (6), p.1044-1047
Main Authors: Singhal, Ritu, Arora, Jyoti, Lal, Prabha, Bhalla, Manpreet, Myneeedu, Vithal, Behera, Digamber
Format: Article
Language:English
Subjects:
Antitubercular Agents - pharmacology
Bacterial Proteins - drug effects
Bacterial Proteins - genetics
Bacteriology
Biological assay
Comparative analysis
Correspondence
Cultures and culture media
Deoxyribonucleic acid
DNA
DNA-Directed RNA Polymerases
Drug resistance in microorganisms
Drug Resistance, Multiple - drug effects
Genes
Genotype & phenotype
Health aspects
Humans
Hybridization
Isoniazid - pharmacology
Laboratories
Microbial Sensitivity Tests - methods
Mutation
Mutation - drug effects
Mycobacterium tuberculosis
Mycobacterium tuberculosis - drug effects
Mycobacterium tuberculosis - genetics
Physiological aspects
Rifampin - pharmacology
Sensitivity and Specificity
Studies
Tuberculosis
Tuberculosis, Multidrug-Resistant - diagnosis
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Summary:Conventional drug susceptibility using solid medium such as Lowenstein - Jensen (L-J) is time consuming whereas liquid medium based methods such as Mycobacterial Growth Indicator Tube (MGIT) System 960 (BD Diagnostics, USA) are sensitive and faster but involve prohibitive expenditure [3] . Commercial line probe assays (LPA) such as INNO-LIPA Rif TB (Innogenetics, Ghent, Belgium) and Genotype MTBDR plus assay (Hain Life Sciences, GmbH, Germany) based on reverse hybridization of amplicons to immobilized membrane based probes covering wild type and common mutation sequences of rpoB and rpoB, katG, inhA respectively, have rapid turn-around time of 48 to 72 h [4] . [...]genotype MTBDR plus assay was found to be rapid, sensitive and specific test for routine drug susceptibility testing for diagnosis of RIF resistance which is more crucial for management of MDR-TB.
ISSN:0971-5916
0975-9174