Increase in Levels of BDNF is Associated with Inflammation and Oxidative Stress during Cardiopulmonary Bypass

• Published in: International journal of biomedical science 2008-09, Vol.4 (3), p.204-211
• Main Authors: Amoureux, Sébastien, Sicard, Pierre, Korandji, Claudia, Borey, Angélique, Benkhadra, Salima, Sequeira-Le Grand, Anabelle, Vergely, Catherine, Girard, Claude, Rochette, Luc
• Format: Article
• Language: English
• ISSN: 1550-9702; 1555-2810
• DOI: 10.59566/IJBS.2008.4204

Cardiopulmonary Bypass (CPB) is thought to generate reactive oxygen species associated with a systemic inflammation and neurotrophins seem to be involved in cardiovascular inflammatory reactions. The aim of this study was to determine the impact of CPB on plasma neurotrophins levels and to appreciate the links existing between inflammation, oxidative stress and neurotrophins. Blood samples were taken from 27 patients undergoing cardiac surgery: before CPB, during ischemia and at reperfusion under CPB. Oxidative stress was evaluated using an Electron Spin Resonance technique by superoxide detection, and antioxidant defences by measurement of Endogenous Peroxidase Activity (EPA). The evolution of two neurotrophins: Brain Derived Neurotrophic Factor (BDNF) and Nerve Growth Factor (NGF) was assessed with an ELISA method. An inflammatory index was determined by a multiplex flow cytometry method. The inflammatory index showed that MCP-1, P-selectin, t-PA and interleukins 6, 8 and 10 levels increased during CPB (p