Measurement of total and local bone morphogenetic protein concentration in bone tumours

Bone morphogenetic protein (BMP) has been shown to be one of the significant factors in the prognosis of bone tumours. In normal development BMP induces new bone formation and later takes part in fracture healing, but its function in malignant tumours is not known. In this study the concentration of...

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Bibliographic Details
Published in:International orthopaedics 1997-07, Vol.21 (3), p.188-193
Main Authors: LAITINEN, M, JORTIKKA, L, HALTTUNEN, T, NEVALAINEN, J, AHO, A. J, MARTTINEN, A, LINDHOLM, T. S
Format: Article
Language:English
Subjects:
Adolescent
Adult
Aged
Aged, 80 and over
Biological and medical sciences
Biomarkers, Tumor - metabolism
Blotting, Western
Bone Morphogenetic Proteins - analysis
Bone Neoplasms - metabolism
Bone Neoplasms - pathology
Chondroma - metabolism
Chondroma - pathology
Chondrosarcoma - metabolism
Chondrosarcoma - pathology
Culture Techniques
Diseases of the osteoarticular system
Female
Giant Cell Tumors - metabolism
Giant Cell Tumors - pathology
Humans
Immunohistochemistry
Male
Medical sciences
Middle Aged
Original
Osteoblastoma - metabolism
Osteoblastoma - pathology
Osteosarcoma - metabolism
Osteosarcoma - pathology
Prognosis
Reproducibility of Results
Sensitivity and Specificity
Tumors of striated muscle and skeleton
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Summary:Bone morphogenetic protein (BMP) has been shown to be one of the significant factors in the prognosis of bone tumours. In normal development BMP induces new bone formation and later takes part in fracture healing, but its function in malignant tumours is not known. In this study the concentration of bone morphogenetic protein was measured in primary bone tumours by two methods. Local staining intensity was detected immunohistologically by the avidin-biotin-peroxidase method determining the highest dilution of anti-serum against bovine bone morphogenetic protein. The total amount of BMP in a tumour sample was measured by an enzyme-linked immunosorbent assay technique after digesting the tissue with collagenase to remove proteins from the connective tissue. Immunohistochemical staining showed that bone morphogenetic protein was present in the cytoplasm and in reactive bone formed by malignant cells. The local concentration was highest in the tissue of giant cell tumours compared to chondrosarcoma, osteosarcoma and benign bone tumours. The total amount in malignant bone tumours was 2.4 times higher compared to benign bone tumours.
ISSN:0341-2695
1432-5195
DOI:10.1007/s002640050148