Endocrine-Disrupting Chemicals (EDCs): In Vitro Mechanism of Estrogenic Activation and Differential Effects on ER Target Genes

• Published in: Environmental health perspectives 2013-04, Vol.121 (4), p.459-466
• Main Authors: Li, Yin, Luh, Colin J, Burns, Katherine A, Arao, Yukitomo, Jiang, Zhongliang, Teng, Christina T, Tice, Raymond R, Korach, Kenneth S
• Format: Article
• Language: English
• Subjects: Activated; Activation; Bisphenol A; Bisphenols; Breast cancer; Cell division; Cell Line, Tumor; Chemicals; Deoxyribonucleic acid; DNA; Endocrine disruptors; Endocrine Disruptors - pharmacology; Endocrine system; Endocrine systems; Endosulfan; Environmental Exposure; Environmental Pollutants - pharmacology; Estrogen; Estrogen Receptor alpha - agonists; Estrogen Receptor alpha - genetics; Estrogen Receptor alpha - metabolism; Estrogen Receptor beta - agonists; Estrogen Receptor beta - genetics; Estrogen Receptor beta - metabolism; Estrogens; Flavonoids; Gene expression; Genes; Growth factors; HeLa Cells; Hep G2 Cells; Humans; Insecticides; Ligands; Luciferases - metabolism; Male; Natural products; Pesticides; Phenols; Plasmids; Real-Time Polymerase Chain Reaction; Receptors; Reproductive system; Rodents; Similarity; Studies; Transcription factors
• ISSN: 0091-6765; 1552-9924
• DOI: 10.1289/ehp.1205951

Endocrine-disrupting chemicals (EDCs) influence the activity of estrogen receptors (ERs) and alter the function of the endocrine system. However, the diversity of EDC effects and mechanisms of action are poorly understood. We examined the agonistic activity of EDCs through ERα and ERβ. We also investigated the effects of EDCs on ER-mediated target genes. HepG2 and HeLa cells were used to determine the agonistic activity of EDCs on ERα and ERβ via the luciferase reporter assay. Ishikawa cells stably expressing ERα were used to determine changes in endogenous ER target gene expression by EDCs. Twelve EDCs were categorized into three groups on the basis of product class and similarity of chemical structure. As shown by luciferase reporter analysis, the EDCs act as ER agonists in a cell type- and promoter-specific manner. Bisphenol A, bisphenol AF, and 2-2-bis(p-hydroxyphenyl)-1,1,1-trichloroethane (group 1) strongly activated ERα estrogen responsive element (ERE)-mediated responses. Daidzein, genistein, kaempferol, and coumestrol (group 2) activated both ERα and ERβ ERE-mediated activities. Endosulfan and kepone (group 3) weakly activated ERα. Only a few EDCs significantly activated the "tethered" mechanism via ERα or ERβ. Results of real-time polymerase chain reaction indicated that bisphenol A and bisphenol AF consistently activated endogenous ER target genes, but the activities of other EDCs on changes of ER target gene expression were compound specific. Although EDCs with similar chemical structures (in the same group) tended to have comparable ERα and ERβ ERE-mediated activities, similar chemical structure did not correlate with previously reported ligand binding affinities of the EDCs. Using ERα-stable cells, we observed that EDCs differentially induced activity of endogenous ER target genes.