In Vivo Determination of Vitamin D Function Using Transgenic Mice Carrying a Human Osteocalcin Luciferase Reporter Gene

Vitamin D is an essential factor for ossification, and its deficiency causes rickets. Osteocalcin, which is a noncollagenous protein found in bone matrix and involved in mineralization and calcium ion homeostasis, is one of the major bone morphogenetic markers and is used in the evaluation of osteob...

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Bibliographic Details
Published in:BioMed research international 2013-01, Vol.2013 (2013), p.1-6
Main Authors: Sato, Kenzo, Yasunaga, Mayu, Soma, Atsumi, Oda, Haruka, Taniguchi, Makoto, Saito, Rumiko, Nakanishi, Tomoko, Yamane, Mariko
Format: Article
Language:English
Subjects:
Animals
Bioluminescence
Calcifediol - metabolism
Genes, Reporter
Humans
Luciferases
Mice
Mice, Transgenic
Osteoblasts - cytology
Osteoblasts - metabolism
Osteocalcin - genetics
Osteocalcin - metabolism
Osteogenesis
Promoter Regions, Genetic
Vitamin D - genetics
Vitamin D - metabolism
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Summary:Vitamin D is an essential factor for ossification, and its deficiency causes rickets. Osteocalcin, which is a noncollagenous protein found in bone matrix and involved in mineralization and calcium ion homeostasis, is one of the major bone morphogenetic markers and is used in the evaluation of osteoblast maturation and osteogenic activation. We established transgenic mouse line expressing luciferase under the control of a 10-kb osteocalcin enhancer/promoter sequence. Using these transgenic mice, we evaluated the active forms of vitamins D2 and D3 for their bone morphogenetic function by in vivo bioluminescence. As the result, strong activity for ossification was observed with 1α,25-hydroxyvitamin D3. Our mouse system can offer a feasible detection method for assessment of osteogenic activity in the development of functional foods and medicines by noninvasive screening.
ISSN:2314-6133
2314-6141
DOI:10.1155/2013/895706