Evaluation of heterogeneous metabolic profile in an orthotopic human glioblastoma xenograft model using compressed sensing hyperpolarized 3D 13C magnetic resonance spectroscopic imaging

• Published in: Magnetic resonance in medicine 2013-07, Vol.70 (1), p.33-39
• Main Authors: Park, Ilwoo, Hu, Simon, Bok, Robert, Ozawa, Tomoko, Ito, Motokazu, Mukherjee, Joydeep, Phillips, Joanna J., James, C. David, Pieper, Russell O., Ronen, Sabrina M., Vigneron, Daniel B., Nelson, Sarah J.
• Format: Article
• Language: English
• Subjects: Animals; Biomarkers, Tumor - metabolism; Brain Neoplasms - metabolism; Carbon Isotopes; Cell Line, Tumor; compressed sensing; Data Compression - methods; dynamic nuclear polarization; glioblastoma; Glioblastoma - metabolism; Humans; hyperpolarized 13C MRSI; Imaging, Three-Dimensional - methods; Magnetic Resonance Imaging - methods; Magnetic Resonance Spectroscopy - methods; Male; Molecular Imaging - methods; Neoplasm Proteins - metabolism; pyruvate; Rats; Rats, Nude; Reproducibility of Results; Sensitivity and Specificity; Tissue Distribution
• ISSN: 0740-3194; 1522-2594
• DOI: 10.1002/mrm.24434

High resolution compressed sensing hyperpolarized 13C magnetic resonance spectroscopic imaging was applied in orthotopic human glioblastoma xenografts for quantitative assessment of spatial variations in 13C metabolic profiles and comparison with histopathology. A new compressed sensing sampling design with a factor of 3.72 acceleration was implemented to enable a factor of 4 increase in spatial resolution. Compressed sensing 3D 13C magnetic resonance spectroscopic imaging data were acquired from a phantom and 10 tumor‐bearing rats following injection of hyperpolarized [1‐13C]‐pyruvate using a 3T scanner. The 13C metabolic profiles were compared with hematoxylin and eosin staining and carbonic anhydrase 9 staining. The high‐resolution compressed sensing 13C magnetic resonance spectroscopic imaging data enabled the differentiation of distinct 13C metabolite patterns within abnormal tissues with high specificity in similar scan times compared to the fully sampled method. The results from pathology confirmed the different characteristics of 13C metabolic profiles between viable, non‐necrotic, nonhypoxic tumor, and necrotic, hypoxic tissue. Magn Reson Med, 2013. © 2012 Wiley Periodicals, Inc.