Characterisation of a monoclonal antibody detecting Atlantic salmon endothelial and red blood cells, and its association with the infectious salmon anaemia virus cell receptor

Endothelial cells (ECs) line the luminal surfaces of the cardiovascular system and play an important role in cardiovascular functions such as regulation of haemostasis and vasomotor tone. A number of fish and mammalian viruses target these cells in the course of their infection. Infectious salmon an...

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Bibliographic Details
Published in:Journal of anatomy 2013-05, Vol.222 (5), p.547-557
Main Authors: Aamelfot, Maria, Weli, Simon C., Dale, Ole B., Koppang, Erling O., Falk, Knut
Format: Article
Language:English
Subjects:
Animals
Antibodies, Monoclonal
Aquaculture
cardiovascular
circulatory
Endothelial Cells - cytology
Endothelial Cells - immunology
erythrocyte
Erythrocytes - cytology
Erythrocytes - immunology
Fish
Fish Diseases - immunology
Fish Diseases - virology
Immunohistochemistry
infectious salmon anaemia virus
Infectious salmon anemia virus
Isavirus - immunology
marker
Original
Receptors, Virus - immunology
Salmo salar
Salmo salar - virology
Salmon
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Summary:Endothelial cells (ECs) line the luminal surfaces of the cardiovascular system and play an important role in cardiovascular functions such as regulation of haemostasis and vasomotor tone. A number of fish and mammalian viruses target these cells in the course of their infection. Infectious salmon anaemia virus (ISAV) attacks ECs and red blood cells (RBCs) of farmed Atlantic salmon (Salmo salar L.), producing the severe disease of infectious salmon anaemia (ISA). The investigation of ISA has up to now been hampered by the lack of a functional marker for ECs in Atlantic salmon in situ. In this study, we report the characterisation and use of a novel monoclonal antibody (MAb) detecting Atlantic salmon ECs (e.g. vessel endothelium, endocardial cells and scavenger ECs) and RBCs. The antibody can be used with immunohistochemistry, IFAT and on Western blots. It appears that the epitope recognised by the antibody is associated with the ISAV cellular receptor. Besides being a tool to identify ECs in situ, it could be useful in further studies of the pathogenicity of ISA. Finally, the detection of an epitope shared by ECs and RBCs agrees with recent findings that these cells share a common origin, thus the MAb can potentially be used to study the ontogeny of these cells in Atlantic salmon.
ISSN:0021-8782
1469-7580
DOI:10.1111/joa.12033