Intestinal Protective Effects of Herbal-Based Formulations in Rats against Neomycin Insult

Disturbance in the gut microbial niche by antibiotics like neomycin produces gastrointestinal (GI) disorders. Here, we evaluated the impact of a mixture of extracts of three herbs (Atractylodis Rhizoma Macrocephalae, Massa Medicata Fermentata, and Dolichoris Semen) with known GI protective activitie...

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Bibliographic Details
Published in:Evidence-based complementary and alternative medicine 2013-01, Vol.2013 (2013), p.1-13
Main Authors: Kim, Hojun, Lee, Myeong-Jong, Han, Kyung-Wan, Bose, Shambhunath
Format: Article
Language:English
Subjects:
Antibiotics
Antimicrobial agents
Bacteria
C-reactive protein
Cecum
Colon
Combined treatment
Diarrhea
Fermentation
Food products
Formulations
Herbs
Histology
Infections
Infectious diseases
Interferon
Intestinal microflora
Intestine
Laboratories
Lactobacillus
Mammals
Medical research
Microbiota
Neomycin
Permeability
Pharmacy
Probiotics
Rats
Water content
Water treatment
Zonula occludens-1 protein
γ-Interferon
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Summary:Disturbance in the gut microbial niche by antibiotics like neomycin produces gastrointestinal (GI) disorders. Here, we evaluated the impact of a mixture of extracts of three herbs (Atractylodis Rhizoma Macrocephalae, Massa Medicata Fermentata, and Dolichoris Semen) with known GI protective activities, either laboratory unfermented (herbal formulation-1 (HF-1)) or fermented/re-fermented (herbal formulation-2 (HF-2)) on neomycin-treated rats using a commercial Lactobacillus probiotic as a reference. Treatment with neomycin augmented stool water content, decreased fecal population of Lactobacillus spp., changed the histology of intestine without inducing inflammation, reduced the colonic expression of zonula occludens-1 (ZO-1) and claudin-1, and elevated the serum C-reactive protein (CRP) and interferon-gamma (IFN-γ) levels. Coadministration of either HF-2 or probiotic, but not HF-1, restored the fecal content of Lactobacillus spp., normalized the serum CRP level, and significantly increased the colonic expression of ZO-1 and claudin-1 in neomycin-treated rats. The combined treatment with any of the above agents ameliorated the histological changes of cecum and colon in neomycin-treated rats, and the magnitude of this effect was probiotic > HF-2 > HF-1. Our study revealed the intestinal protective effect of a mixture of three herbs against neomycin insult, which is mediated through multiple mechanisms and is potentiated upon prior fermentation/refermentation of the herbs.
ISSN:1741-427X
1741-4288
DOI:10.1155/2013/161278