Heterozygous alleles restore male fertility to cytoplasmic male-sterile radish (Raphanus sativus L.): a case of overdominance

The practice of hybridization has greatly contributed to the increase in crop productivity. A major component that exploits heterosis in crops is the cytoplasmic male sterility (CMS)/nucleus-controlled fertility restoration (Rf) system. Through positional cloning, it is shown that heterozygous allel...

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Bibliographic Details
Published in:Journal of experimental botany 2013-04, Vol.64 (7), p.2041-2048
Main Authors: Wang, Zhi Wei, Wang, Chuan, Gao, Lei, Mei, Shi Yong, Zhou, Yuan, Xiang, Chang Ping, Wang, Ting
Format: Article
Language:English
Subjects:
Agronomy. Soil science and plant productions
Alleles
Anthers
Biological and medical sciences
crops
Cytoplasmic male sterility
DNA-directed RNA polymerase
Fundamental and applied biological sciences. Psychology
Gene Expression Regulation, Plant
Gene Expression Regulation, Plant - genetics
Gene Expression Regulation, Plant - physiology
Genes, Dominant
Genetic loci
Genetics
Genetics and breeding of economic plants
Genomes
heterosis
Heterosis. Floral biology applications: apomixy, male sterility, incompatibility, varia
heterozygosity
Heterozygote
homozygosity
hybridization
Male fertility
metabolism
molecular cloning
overdominance
physiology
Plant breeding: fundamental aspects and methodology
Plant fertility
Plant Infertility
Plant Infertility - genetics
Plant Infertility - physiology
Plant physiology and development
Plant Proteins
Plant Proteins - genetics
Plant Proteins - metabolism
Plants
proteins
Radishes
Raphanus
Raphanus - genetics
Raphanus sativus
RESEARCH PAPER
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Summary:The practice of hybridization has greatly contributed to the increase in crop productivity. A major component that exploits heterosis in crops is the cytoplasmic male sterility (CMS)/nucleus-controlled fertility restoration (Rf) system. Through positional cloning, it is shown that heterozygous alleles (RsRf3-1/RsRf3-2) encoding pentatricopeptide repeat (PPR) proteins are responsible for restoring fertility to cytoplasmic male-sterile radish (Raphanus sativus L.). Furthermore, it was found that heterozygous alleles (RsRf3-1/RsRf3-2) show higher expression and RNA polymerase II occupancy in the CMS cytoplasmic background compared with their homozygous alleles (RsRf3-1/RsRf3-1 or RsRf3-2/RsRf3-2). These data provide new insights into the molecular mechanism of fertility restoration to cytoplasmic male-sterile plants and illustrate a case of overdominance.
ISSN:0022-0957
1460-2431
DOI:10.1093/jxb/ert065