BMSCs reduce rat granulosa cell apoptosis induced by cisplatin and perimenopause

The objective of this study was to evaluate the effect of bone marrow mesenchymal stem cells (BMSCs) on the apoptosis of granulosa cells (GCs) in rats. Cisplatin increased GC apoptosis from 0.59% to 13.04% in the control and cisplatin treatment groups, respectively, which was significantly reduced u...

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Published in:BMC cell biology 2013-03, Vol.14 (1), p.18-18, Article 18
Main Authors: Guo, Jun-Qi, Gao, Xia, Lin, Zhi-Jie, Wu, Wei-Zhen, Huang, Liang-Hu, Dong, Hui-Yue, Chen, Jin, Lu, Jun, Fu, Yun-Fen, Wang, Jin, Ma, Yu-Jie, Chen, Xiao-Wen, Wu, Zhi-Xian, He, Fu-Qiang, Yang, Shun-Liang, Liao, Lian-Ming, Zheng, Feng, Tan, Jian-Ming
Format: Article
Language:English
Subjects:
Animals
Apoptosis
Apoptosis - drug effects
bcl-2-Associated X Protein - genetics
bcl-2-Associated X Protein - metabolism
Bone Marrow Cells - drug effects
Bone Marrow Cells - physiology
Cells, Cultured
Cisplatin
Cisplatin - administration & dosage
Coculture Techniques - methods
Cyclin-Dependent Kinase Inhibitor p21 - genetics
Cyclin-Dependent Kinase Inhibitor p21 - metabolism
Estrogen
Estrogens - administration & dosage
Female
Flow cytometry
Gene Expression Regulation - drug effects
Granulosa Cells - drug effects
Granulosa Cells - physiology
Menopause
Mesenchymal Stem Cell Transplantation
Mesenchymal Stromal Cells - drug effects
Mesenchymal Stromal Cells - physiology
Microscopy
Perimenopause - physiology
Proto-Oncogene Proteins c-myc - genetics
Proto-Oncogene Proteins c-myc - metabolism
Rats
Rats, Sprague-Dawley
RNA
Rodents
Statistical analysis
Stem cells
Studies
Transplants & implants
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Summary:The objective of this study was to evaluate the effect of bone marrow mesenchymal stem cells (BMSCs) on the apoptosis of granulosa cells (GCs) in rats. Cisplatin increased GC apoptosis from 0.59% to 13.04% in the control and cisplatin treatment groups, respectively, which was significantly reduced upon co-culture with BMSCs to 4.84%. Cisplatin treatment increased p21 and bax and decreased c-myc mRNA expression, which was reversed upon co-culture with BMSCs. As compared to young rats, increased apoptosis was observed in the perimenopausal rats (P 
ISSN:1471-2121
1471-2121
DOI:10.1186/1471-2121-14-18