Metal‐Conjugated Affinity Labels: A New Concept to Create Enantioselective Artificial Metalloenzymes

How to train a protein: Metal‐conjugated affinity labels were used to selectively position catalytically active metal centers in the binding pocket of proteases. The resulting artificial metalloenzymes achieve up to 82 % e.r. in the hydrogenation of ketones. The modular setup enables a rapid generat...

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Bibliographic Details
Published in:ChemistryOpen (Weinheim) 2013-04, Vol.2 (2), p.50-54
Main Authors: Reiner, Thomas, Jantke, Dominik, Marziale, Alexander N., Raba, Andreas, Eppinger, Jörg
Format: Article
Language:English
Subjects:
Affinity
affinity labels
artificial metalloproteins
asymmetric catalysis
Binding
Catalysis
Communications
cysteine proteases
Enantiomers
Hydrogenation
Labels
Modular
Pocket
Trains
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Summary:How to train a protein: Metal‐conjugated affinity labels were used to selectively position catalytically active metal centers in the binding pocket of proteases. The resulting artificial metalloenzymes achieve up to 82 % e.r. in the hydrogenation of ketones. The modular setup enables a rapid generation of artificial metalloenzyme libraries, which can be adapted to a broad range of catalytic conditions.
ISSN:2191-1363
2191-1363
DOI:10.1002/open.201200044