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The Pivotal Role of IKKα in the Development of Spontaneous Lung Squamous Cell Carcinomas
Here, we report that kinase-dead IKKα knockin mice develop spontaneous lung squamous cell carcinomas (SCCs) associated with IKKα downregulation and marked pulmonary inflammation. IKKα reduction upregulated the expression of p63, Trim29, and keratin 5 (K5), which serve as diagnostic markers for human...
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Published in: | Cancer cell 2013-04, Vol.23 (4), p.527-540 |
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Main Authors: | , , , , , , , , , , , , , , , , , , , , |
Format: | Article |
Language: | English |
Subjects: | |
Citations: | Items that this one cites Items that cite this one |
Online Access: | Get full text |
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Summary: | Here, we report that kinase-dead IKKα knockin mice develop spontaneous lung squamous cell carcinomas (SCCs) associated with IKKα downregulation and marked pulmonary inflammation. IKKα reduction upregulated the expression of p63, Trim29, and keratin 5 (K5), which serve as diagnostic markers for human lung SCCs. IKKαlowK5+p63hi cell expansion and SCC formation were accompanied by inflammation-associated deregulation of oncogenes, tumor suppressors, and stem cell regulators. Reintroducing transgenic K5.IKKα, depleting macrophages, and reconstituting irradiated mutant animals with wild-type bone marrow (BM) prevented SCC development, suggesting that BM-derived IKKα mutant macrophages promote the transition of IKKαlowK5+p63hi cells to tumor cells. This mouse model resembles human lung SCCs, sheds light on the mechanisms underlying lung malignancy development, and identifies targets for therapy of lung SCCs.
► We establish a robust mouse lung SCC model that resembles human lung SCCs ► IKKα reduction deregulates oncogenes, tumor suppressors, and stem cell genes ► Increased IKKα mutant macrophages promote the initiation of lung SCCs ► IKKα prevents the abnormality of squamous cells in multiple epithelial organs |
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ISSN: | 1535-6108 1878-3686 |
DOI: | 10.1016/j.ccr.2013.03.009 |