Oxidative Stress and Free-Radical Oxidation in BCG Granulomatosis Development

Background. Little is known about the role of free-radical and oxidative stress signaling in granuloma maturation and resolution. We aimed to study the activity of free-radical oxidation processes in the dynamics of BCG-induced generalized granulomatosis in mice. Methods. Chronic granulomatous infla...

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Published in:Oxidative medicine and cellular longevity 2013-01, Vol.2013 (2013), p.1-8
Main Authors: Menshchikova, Elena, Zenkov, Nikolay, Tkachev, Victor, Potapova, Oksana, Cherdantseva, Liliya, Shkurupiy, Vyacheslav A.
Format: Article
Language:English
Subjects:
Animals
BCG Vaccine - adverse effects
Body Weight
Cell Count
Fluorescence
Free Radicals - metabolism
Granuloma - etiology
Granuloma - pathology
Hydrogen Peroxide - metabolism
Liver - pathology
Luminescent Measurements
Luminol - metabolism
Lung - pathology
Macrophages - pathology
Male
Mice
Mice, Inbred BALB C
Neutrophils - pathology
Organ Size
Oxidation-Reduction
Oxidative Stress
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Summary:Background. Little is known about the role of free-radical and oxidative stress signaling in granuloma maturation and resolution. We aimed to study the activity of free-radical oxidation processes in the dynamics of BCG-induced generalized granulomatosis in mice. Methods. Chronic granulomatous inflammation was induced in male BALB/c mice by intravenously injecting the BCG vaccine, and the production of oxidative stress (activity of free-radical oxidation processes) and histological changes in the lungs, liver, and peritoneal exudate were measured 3, 30, 60, and 90 days after infection. Results. The tuberculous granuloma numerical density and diameter continuously increased from day 30 to day 90, and the macrophage content within the granulomas progressively diminished with a concomitant elevation in the number of epithelioid cells. The activity of the free-radical oxidation processes in the liver (i.e., the intensity of the homogenate chemiluminescence) reached a maximum at postinfection day 60 and subsequently began to decrease. The peak generation of reactive oxygen species by phagocytes in the peritoneal exudate (measured using flow cytometry) was also shifted in time and fell on day 30. Conclusions. The rise in the steady-state concentration of H2O2 in the liver of mice with BCG-induced granulomatosis is not related to local H2O2 production by phagocytes, and a decrease in the severity of generalized inflammation precedes the resolution of local inflammation.
ISSN:1942-0900
1942-0994
DOI:10.1155/2013/452546