Revealing the Mechanism of In Vitro Wound Healing Properties of Citrus tamurana Extract

• Published in: BioMed research international 2013-01, Vol.2013 (2013), p.1-8
• Main Authors: Ikenoue, Tsuyomu, Sameshima, Hiroshi, Masatoshi, Yamaguchi, Harishkumar, Madhyastha, Masugi, Maruyama
• Format: Article
• Language: English
• Subjects: Cell Death - drug effects; Cell Division - drug effects; Cell Line; Cell Movement - drug effects; Cell Proliferation - drug effects; Citrus - chemistry; Cyclin-Dependent Kinases - genetics; Cyclin-Dependent Kinases - metabolism; Fibroblasts - drug effects; Fibroblasts - enzymology; Fibroblasts - pathology; G2 Phase - drug effects; Gene Expression Regulation - drug effects; Humans; Plant Extracts - pharmacology; Protein Biosynthesis - drug effects; rho GTP-Binding Proteins - genetics; rho GTP-Binding Proteins - metabolism; RNA, Messenger - genetics; RNA, Messenger - metabolism; Transcription, Genetic - drug effects; Wound Healing - drug effects
• ISSN: 2314-6133; 2314-6141
• DOI: 10.1155/2013/963457

In the present investigation, we examined the effect of Hyuganatsu (Citrus tamurana) extract (HE) on skin fibroblast (TIG-119) proliferation and migration during in vitro wound healing. HE selectively inhibited proliferation of TIG-119 cells at higher concentration (>1.0 mg/mL); at lower concentrations (0.1, 0.25, 0.5, and 0.75 mg/mL), it exhibited linear and time-dependent cell proliferation. In vitro scratch wound healing studies showed that the HE also accelerated the migration of cells towards the wounded region. Cytometric analysis demonstrated that HE extract did not alter G1/0 and S phases of cell cycle in any concentration studied; however, G2/M phases of cell cycle were significantly (P