The Role of Allosteric Coupling on Thermal Activation of Thermo-TRP Channels

Thermo-transient receptor potential channels display outstanding temperature sensitivity and can be directly gated by low or high temperature, giving rise to cold- and heat-activated currents. These constitute the molecular basis for the detection of changes in ambient temperature by sensory neurons...

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Bibliographic Details
Published in:Biophysical journal 2013-05, Vol.104 (10), p.2160-2169
Main Authors: Jara-Oseguera, Andrés, Islas, León D.
Format: Article
Language:English
Subjects:
Allosteric Regulation
ambient temperature
Animals
Biophysics
Channels and Transporters
Computer Simulation
cooling
enthalpy
Entropy
heat
HEK293 Cells
Humans
Ion Channel Gating
Kinetics
Molecular physics
Neurons
sensory neurons
Sensory perception
Temperature
Temperature effects
Thermodynamics
Transient Receptor Potential Channels - chemistry
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Summary:Thermo-transient receptor potential channels display outstanding temperature sensitivity and can be directly gated by low or high temperature, giving rise to cold- and heat-activated currents. These constitute the molecular basis for the detection of changes in ambient temperature by sensory neurons in animals. The mechanism that underlies the temperature sensitivity in thermo-transient receptor potential channels remains unknown, but has been associated with large changes in standard-state enthalpy (ΔHo) and entropy (ΔSo) upon channel gating. The magnitude, sign, and temperature dependence of ΔHo and ΔSo, the last given by an associated change in heat capacity (ΔCp), can determine a channel’s temperature sensitivity and whether it is activated by cooling, heating, or both, if ΔCp makes an important contribution. We show that in the presence of allosteric gating, other parameters, besides ΔHo and ΔSo, including the gating equilibrium constant, the strength- and temperature dependence of the coupling between gating and the temperature-sensitive transitions, as well as the ΔHo/ΔSo ratio associated with them, can also determine a channel’s temperature-dependent activity, and even give rise to channels that respond to both cooling and heating in a ΔCp-independent manner.
ISSN:0006-3495
1542-0086
DOI:10.1016/j.bpj.2013.03.055